Thalassocella blandensis gen. nov., sp. nov., a novel member of the family Cellvibrionaceae
| Autor: | David R. Arahal, María J. Pujalte, Isabel Sanz-Sáez, Rosa Aznar, Carlos Pedrós-Alió, Silvia G. Acinas, Teresa Lucena, Olga Sánchez |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España) |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Biology 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences Saccharophagus degradans Gammaproteobacteria Magnesium ion Genome size Ecology Evolution Behavior and Systematics Strain (chemistry) Marine bacteria Agarilytica General Medicine AA 16S ribosomal RNA biology.organism_classification Halophile Thalassocella 030104 developmental biology Biochemistry Cellvibrionaceae ANI Teredinibacter Energy source |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname International Journal of Systematic and Evolutionary Microbiology |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | 9 pages, 1 figure, 3 tables Strain ISS155T, isolated from surface Mediterranean seawater, has cells that are Gram-reaction-negative, motile, strictly aerobic chemoorganotrophic, oxidase-positive, unable to reduce nitrate to nitrite, and able to grow with cellulose as the sole carbon and energy source. It is mesophilic, neutrophilic, slightly halophilic and has a requirement for sodium and magnesium ions. Its 16S rRNA gene sequence places the strain among members of Cellvibrionaceae, in the Gammaproteobacteria, with Agarilytica rhodophyticola 017T as closest relative (94.3¿% similarity). Its major cellular fatty acids are C18¿:¿1, C16¿:¿0 and C16¿:¿1; major phospholipids are phosphatidyl glycerol, phosphatidyl ethanolamine and an unidentified lipid, and the major respiratory quinone is Q8. The genome size is 6.09 Mbp and G+C content is 45.2¿mol%. A phylogenomic analysis using UBCG merges strain ISS155T in a clade with A. rhodophyticola, Teredinibacter turnerae, Saccharophagus degradans and Agaribacterium haliotis type strain genomes, all of them possessing a varied array of carbohydrate-active enzymes and the potential for polysaccharide degradation. Average amino acid identity indexes determined against available Cellvibrionaceae type strain genomes show that strain ISS155T is related to them by values lower than 60¿%, with a maximum of 58¿% to A. rhodophyticola 017T and 57¿% to T. turnerae T7902T and S. degradans 2-40T. These results, together with the low 16S rRNA gene sequence similarities and differences in phenotypic profiles, indicate that strain ISS155T represents a new genus and species in Cellvibrionaceae, for which we propose the name Thalassocella blandensis gen. nov., sp. nov., and strain ISS155T (=CECT 9533T=LMG 31237T) as the type strain Sequencing was funded by grant CTM2016-80095-C2-1-R to C.P.A. from the Spanish Ministerio de Economía y Competitividad. Additional funding was obtained by grant CTM2017-87736-R to S.G.A. from the Spanish Ministerio de Economía y Competitividad With the funding support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S), of the Spanish Research Agency (AEI) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|