Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation
| Autor: | Scott P. Kallgren, Dan Spatt, Patrick Cramer, Burak H. Alver, Kerstin C. Maier, Ameet Shetty, Carina Demel, Fred Winston, Peter J. Park |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Time Factors Transcription Elongation Genetic Genotype Chromosomal Proteins Non-Histone RNA Splicing Response element E-box RNA polymerase II Saccharomyces cerevisiae Biology Article 03 medical and health sciences 0302 clinical medicine Sp3 transcription factor Gene Expression Regulation Fungal Databases Genetic Schizosaccharomyces RNA Antisense RNA Messenger Promoter Regions Genetic Molecular Biology Genetics General transcription factor Computational Biology Promoter RNA Fungal Cell Biology Cell biology 030104 developmental biology Phenotype Transcription preinitiation complex TAF2 Mutation biology.protein RNA Polymerase II Schizosaccharomyces pombe Proteins Genome Fungal Transcriptional Elongation Factors 030217 neurology & neurosurgery |
| Zdroj: | Molecular Cell |
| ISSN: | 1097-4164 |
| Popis: | Spt5 is an essential and conserved factor that functions in transcription and co-transcriptional processes. However, many aspects of the requirement for Spt5 in transcription are poorly understood. We have analyzed the consequences of Spt5 depletion in Schizosaccharomyces pombe, using four genome-wide approaches. Our results demonstrate that Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. In the absence of Spt5, RNAPII localization changes dramatically, with reduced levels and a relative accumulation over the first ~500 bp, suggesting that Spt5 is required for transcription past a barrier. Spt5 depletion also results in widespread antisense transcription initiating within this barrier region. Deletions of this region alter the distribution of RNAPII on the sense strand, suggesting that the barrier observed after Spt5 depletion is normally a site at which Spt5 stimulates elongation. Our results reveal a global requirement for Spt5 in transcription elongation. |
| Databáze: | OpenAIRE |
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