Inhibitory effects of omega-3 fatty acids on protein kinase C activity in vitro
| Autor: | A L Stoll, Lauren B. Marangell, H F Seung Kim, Edwin J. Weeber, J.D. Sweatt |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Bipolar Disorder
Docosahexaenoic Acids Protein Kinase C beta Arachidonic Acids In Vitro Techniques Biology Phosphotransferase Cellular and Molecular Neuroscience chemistry.chemical_compound In vivo Fatty Acids Omega-3 Animals Molecular Biology Protein Kinase C Protein kinase C chemistry.chemical_classification Arachidonic Acid Brain Eicosapentaenoic acid Rats Enzyme Activation Psychiatry and Mental health Enzyme chemistry Biochemistry Docosahexaenoic acid lipids (amino acids peptides and proteins) Arachidonic acid |
| Zdroj: | Molecular Psychiatry. 6:246-248 |
| ISSN: | 1476-5578 1359-4184 |
| Popis: | Preliminary clinical data indicate that omega-3 fatty acids may be effective mood stabilizers for patients with bipolar disorder. Both lithium and valproic acid are known to inhibit protein kinase C (PKC) activity after subchronic administration in cell culture and in vivo. The current study was undertaken to determine the effects of the omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on protein kinase C phosphotransferase activity in vitro. Various concentrations of DHA, EPA, and arachidonic acid (AA) were incubated with the catalytic domain of protein kinase C beta from rat brain. Protein kinase C activity was measured by quantifying incorporation of (32)P-PO(4) into a synthetic peptide substrate. Both DHA and EPA, as well as the combination of DHA and EPA, inhibited PKC activity at concentrations as low as 10 micromol l(-1). In contrast, arachidonic acid had no effect on PKC activity. Thus, PKC represents a potential site of action of omega-3 fatty acids in their effects on the treatment of bipolar disorder. |
| Databáze: | OpenAIRE |
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