Characterization of a New Protein Family Associated With the Silica Deposition Vesicle Membrane Enables Genetic Manipulation of Diatom Silica
| Autor: | Mark Hildebrand, Benoit Tesson, Sarah J. L. Lerch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Frustule Protein family Gene Expression lcsh:Medicine 02 engineering and technology Biology Article Cell wall 03 medical and health sciences Genes Reporter Amino Acid Sequence Cytoskeleton lcsh:Science Diatoms Organelles Multidisciplinary Vesicle lcsh:R Membrane Proteins Intracellular Membranes Silicon Dioxide 021001 nanoscience & nanotechnology biology.organism_classification Transmembrane protein Transport protein Protein Transport Transmembrane domain 030104 developmental biology Biochemistry Gene Knockdown Techniques Biophysics lcsh:Q Genetic Engineering 0210 nano-technology |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-13613-8 |
| Popis: | Diatoms are known for their intricate, silicified cell walls (frustules). Silica polymerization occurs in a compartment called the silica deposition vesicle (SDV) and it was proposed that the cytoskeleton influences silica patterning through the SDV membrane (silicalemma) via interactions with transmembrane proteins. In this work we identify a family of proteins associated with the silicalemma, named SAPs for Silicalemma Associated Proteins. The T. pseudonana SAPs (TpSAPs) are characterized by their motif organization; each contains a transmembrane domain, serine rich region and a conserved cytoplasmic domain. Fluorescent tagging demonstrated that two of the TpSAPs were localized to the silicalemma and that the intralumenal region of TpSAP3 remained embedded in the silica while the cytoplasmic region was cleaved. Knockdown lines of TpSAP1 and 3 displayed malformed valves; which confirmed their roles in frustule morphogenesis. This study provides the first demonstration of altering silica structure through manipulation of a single gene. |
| Databáze: | OpenAIRE |
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