Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy
| Autor: | Hui Zhang, Shu Jia, Lianyu Jin, Jianzuo Yao MB, Zhihong Shen, Jingyi Wu, Xiaokun Yao, Danwei Chen, Congcong Zhang, Shufang Yu, Ningwei Zhu, Lexiao Jin, Xiaomin Yao |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Acta Cirúrgica Brasileira, Volume: 36, Issue: 11, Article number: e361104, Published: 18 FEB 2022 Acta Cirúrgica Brasileira v.36 n.11 2021 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
| ISSN: | 1678-2674 0102-8650 |
| Popis: | Purpose: To investigate the underlying mechanism of hepatic sinusoidal obstruction syndrome (HSOS) induced by Gynura segetum by measuring autophagy in mouse models. Methods: The model group was administered G. segetum (30 g/kg/d) by gavage, while the normal control group was administered an equal volume of saline daily for five weeks. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic histopathological examinations, and Masson staining were performed to evaluate liver injury. Liver intercellular adhesion molecule-1 (ICAM-1) and P-selectin were evaluated by immunohistochemistry. Hepatocellular apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Protein expression levels of autophagy markers were measured using Western blot analysis. Results: Gynura segetum was found to significantly induce liver injury compared with control mice, as evidenced by the increase of serum transaminases, a decrease in triglyceride levels, and histopathological changes in mice. Gynura segetum remarkably induced hepatocellular apoptosis and upregulated the expressions of ICAM-1 and P-selectin and also downregulated the protein expression levels of LC3, Atg12 and cytoplasmic polyadenylation element binding protein. Conclusions: Our results suggested that G. segetum induced liver injury with HSOS, and it was partly due to its ability to impair the autophagy pathway. |
| Databáze: | OpenAIRE |
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