Liraglutide Exerts Protective Effects by Downregulation of PPARγ, ACSL1 and SREBP-1c in Huh7 Cell Culture Models of Non-Alcoholic Steatosis and Drug-Induced Steatosis
| Autor: | Tea Omanovic Kolaric, Tomislav Kizivat, Vjera Mihaljevic, Milorad Zjalic, Ines Bilic-Curcic, Lucija Kuna, Robert Smolic, Aleksandar Vcev, George Y. Wu, Martina Smolic |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences liraglutide BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti BIOMEDICINE AND HEALTHCARE. Basic Medical Sciences NAS amiodarone Huh7 cell line General Medicine Microbiology BIOMEDICINA I ZDRAVSTVO. Temeljne medicinske znanosti Molecular Biology |
| Zdroj: | Current Issues in Molecular Biology Volume 44 Issue 8 Current Issues in Molecular Biology; Volume 44; Issue 8; Pages: 3465-3480 |
| ISSN: | 1467-3045 |
| DOI: | 10.3390/cimb44080239 |
| Popis: | (1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug-induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 µM AMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARγ, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS. |
| Databáze: | OpenAIRE |
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