Functional characterization and expression of GASCL1 and GASCL2, two anther-specific chalcone synthase like enzymes from Gerbera hybrida

Autor: Juha Kontturi, Victor A. Albert, Xianbao Deng, Hany Bashandy, Teemu H. Teeri, Raisa Osama
Přispěvatelé: University of Helsinki, Department of Agricultural Sciences, Teemu Teeri / Principal Investigator, Viikki Plant Science Centre (ViPS), Asteraceae developmental biology and secondary metabolism, Plant Production Sciences
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Gerbera hybrida
SPOROPOLLENIN BIOSYNTHESIS
Plant Science
Asteraceae
01 natural sciences
Biochemistry
PATHWAY
chemistry.chemical_compound
Biopolymers
Gene Expression Regulation
Plant
ASCL
Arabidopsis thaliana
1183 Plant biology
microbiology
virology
ANTHOCYANIN BIOSYNTHESIS
biology
III POLYKETIDE SYNTHASES
food and beverages
General Medicine
Recombinant Proteins
ASTERACEAE INFLORESCENCE
Sporopollenin
Pollen
DIVERSIFICATION
Chalcone synthase
Gerbera
Chalcone
GENES
Protein family
Type III polyketide synthase
Flowers
Horticulture
Genes
Plant
POLLEN EXINE DEVELOPMENT
03 medical and health sciences
Biosynthesis
PLANTS
Molecular Biology
biology.organism_classification
Carotenoids
030104 developmental biology
Flavonoid biosynthesis
chemistry
Pyrones
ARABIDOPSIS-THALIANA
biology.protein
1182 Biochemistry
cell and molecular biology
Acyl Coenzyme A
Polyketide Synthases
Acyltransferases
010606 plant biology & botany
Zdroj: Phytochemistry. 134:38-45
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2016.11.002
Popis: The chalcone synthase superfamily consists of type III polyketidesynthases (PKSs), enzymes responsible for producing plant secondary metabolites with various biological and pharmacological activities. Anther-specific chalcone synthase-like enzymes (ASCLs) represent an ancient group of type III PKSs involved in the biosynthesis of sporopollenin, the main component of the exine layer of moss spores and mature pollen grains of seed plants. In the latter, ASCL proteins are localized in the tapetal cells of the anther where they participate in sporopollenin biosynthesis and exine formation within the locule. It is thought that the enzymes responsible for sporopollenin biosynthesis are highly conserved, and thus far, each angiosperm species with a genome sequenced has possessed two ASCL genes, which in Arabidopsis thaliana are PKSA and PKSB. The Gerbera hybrida (gerbera) PKS protein family consists of three chalcone synthases (GCHS1, GCHS3 and GCHS4) and three 2-pyrone synthases (G2PS1, G2PS2 and G2PS3). In previous studies we have demonstrated the functions of chalcone synthases in flavonoid biosynthesis, and the involvement of 2-pyrone synthases in the biosynthesis of antimicrobial compounds found in gerbera. In this study we expanded the gerbera PKS-family by functionally characterizing two gerbera ASCL proteins. In vitro enzymatic studies using purified recombinant proteins showed that both GASCL1 and GASCL2 were able to use medium and long-chain acyl-CoA starters and perform two to three condensation reactions of malonyl-CoA to produce tri- and tetraketide 2-pyrones, usually referred to as alpha-pyrones in sporopollenin literature. Both GASCL1 and GASCL2 genes were expressed only floral organs, with most expression observed in anthers. In the anthers, transcripts of both genes showed strict tapetum-specific localization. (C) 2016 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
Externí odkaz: