Alpha(2) macroglobulin, a PSA binding protein, is expressed in human prostate stroma
| Autor: | Claus G. Roehrborn, John D. McConnell, Victor K. Lin, Nicholas C. Boetticher, Hossein Saboorian, Dolores V. Vazquez, Shih-Ya Wang |
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| Rok vydání: | 2004 |
| Předmět: |
PCA3
Male Stromal cell Urology Blotting Western Prostatic Hyperplasia Alpha (ethology) Gene Expression In situ hybridization Biology Tissue Culture Techniques Prostate medicine Humans alpha-Macroglobulins RNA Messenger Cells Cultured In Situ Hybridization Differential display Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Molecular biology Immunohistochemistry Macroglobulin medicine.anatomical_structure Oncology Culture Media Conditioned Electrophoresis Polyacrylamide Gel Stromal Cells |
| Zdroj: | The Prostate. 63(3) |
| ISSN: | 0270-4137 |
| Popis: | BACKGROUND Benign prostatic hyperplasia (BPH) is characterized as a stromal process. The stroma smooth muscle (SM) may alter its phenotype during the progression of BPH. We have identified gene transcripts that may be differentially expressed in BPH using a differential display method. Among the fragments isolated, alpha(2) macroglobulin (alpha(2)-M) is one of the most interesting. alpha(2)-M is a binding protein of a variety of proteinases, including prostatic specific antigen (PSA). It also plays roles in molecular trapping and targeting. In this study, we characterized alpha(2)-M expression in the human prostate. METHODS Differential display was used to identify and isolate the differentially expressed transcripts between normal prostate and BPH tissues. RT-PCR, Western blot, in situ hybridization, and immunohistochemistry were utilized to confirm and characterize alpha(2)-M expression in the prostate. RESULTS Real-time RT-PCR results revealed that a 3.2-fold increase in alpha(2)-M mRNA expression is observed in BPH compared with normal prostate tissue. A 1.9-fold increase at protein level was also observed. In situ hybridization and immunohistochemistry showed that alpha(2)-M expression is primarily localized to the stromal compartment. Cultured primary stroma cells maintained alpha(2)-M expression, while prostate epithelial cells had a significantly lower level of alpha(2)-M expression. Furthermore, stromal cells in culture produce and secrete alpha(2)-M in the medium. CONCLUSIONS We identified alpha(2)-M expression in the human prostate. An increased alpha(2)-M expression appears to be associated with BPH. Considering the unique features of its protein binding and targeting properties, alpha(2)-M expressed in the prostate may play an important role in regulating benign and malignant prostatic growth. |
| Databáze: | OpenAIRE |
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