Accurate point-of-care serology tests for COVID-19
| Autor: | Jonathan P. Troost, James L. Baldwin, James R. Baker, Don Giacherio, Carmen Gherasim, Charles F. Schuler, Jesse Chen, Kelly O'Shea, David M Manthei |
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| Rok vydání: | 2021 |
| Předmět: |
RNA viruses
Male 0301 basic medicine Viral Diseases Pulmonology Physiology Coronaviruses Antibodies Viral Biochemistry Serology Medical Conditions Electronics Engineering 0302 clinical medicine Immune Physiology Medicine and Health Sciences Medicine 030212 general & internal medicine Pathology and laboratory medicine Virus Testing Immunoassay Immune System Proteins Multidisciplinary medicine.diagnostic_test biology Medical microbiology Middle Aged Body Fluids Infectious Diseases Blood Comparators Viruses Engineering and Technology Female Anatomy SARS CoV 2 Pathogens Antibody Research Article Adult SARS coronavirus Coronavirus disease 2019 (COVID-19) Point-of-Care Systems Science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Research and Analysis Methods Microbiology Sensitivity and Specificity Antibodies Virus COVID-19 Serological Testing Respiratory Disorders Young Adult 03 medical and health sciences Antigen Diagnostic Medicine Humans Immunoassays Nucleocapsid Aged Point of care SARS-CoV-2 business.industry Organisms Viral pathogens Biology and Life Sciences Proteins COVID-19 Covid 19 Microbial pathogens 030104 developmental biology Immunoglobulin M Immunoglobulin G Respiratory Infections Immunologic Techniques biology.protein Reagent Kits Diagnostic Electronics business |
| Zdroj: | PLoS ONE, Vol 16, Iss 3, p e0248729 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background As COVID-19 vaccines become available, screening individuals for prior COVID-19 infection and vaccine response in point-of-care (POC) settings has renewed interest. We prospectively screened at-risk individuals for SARS-CoV-2 spike and nucleocapsid protein antibodies in a POC setting to determine if it was a feasible method to identify antibody from prior infection. Methods Three EUA-approved lateral flow antibody assays were performed on POC finger-stick blood and compared with serum and a CLIA nucleocapsid antibody immunoassay. Variables including antibody class, time since PCR, and the assay antigen used were evaluated. Results 512 subjects enrolled, of which 104 had a COVID-19 history and positive PCR. Only three PCR-positive subjects required hospitalization, with one requiring mechanical ventilation. The POC results correlated well with the immunoassay (93–97% sensitivity) and using serum did not improve the sensitivity or specificity. Conclusions Finger-stick, POC COVID-19 antibody testing was highly effective in identifying antibody resulting from prior infections in mildly symptomatic subjects. Using high-complexity serum immunoassays did not improve the screening outcome. Almost all individuals with COVID-19 infection produced detectable antibodies to the virus. POC antibody testing is useful as a screen for prior COVID-19 infection, and should be useful in assessing vaccine response. |
| Databáze: | OpenAIRE |
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