Estrogenic effects following larval exposure to the putative anti-estrogen, fulvestrant, in the fathead minnow ( Pimephales promelas )
| Autor: | Alan S. Kolok, Daniel D. Snow, Edwin Chavez, Jonathan Ramirez, Sathaporn Onanong, Michael T. Palandri, Jonathan M. Ali, Alex T. Kallenbach |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Gonad Physiology Secondary sex characteristic Health Toxicology and Mutagenesis Cyprinidae Disorders of Sex Development 010501 environmental sciences Biology Toxicology 01 natural sciences Biochemistry Andrology 03 medical and health sciences Internal medicine biology.animal medicine Animals Sexual maturity Sex Ratio Fulvestrant 0105 earth and related environmental sciences Sexual differentiation Dose-Response Relationship Drug Estradiol Gene Expression Regulation Developmental Cell Biology General Medicine Minnow 030104 developmental biology Endocrinology medicine.anatomical_structure Larva Female Estrogen Receptor Antagonists Sex ratio Sex characteristics medicine.drug |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 204:26-35 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2017.10.013 |
| Popis: | The objective of the present study was to investigate the consequences of early-life exposure to fulvestrant on estrogenic gene expression in fathead minnow larvae. To address this objective, fathead minnow larvae were exposed to fulvestrant (ICI 182,780) during the window of sexual differentiation between 0 to 30 days post-hatch (dph). The four treatment groups in this study included: filtered water controls (never exposed), solvent controls (ethanol 0.01%), and nominally low (0.10μg/L) and high (10.0μg/L) doses of fulvestrant. Following 30 d exposure to their respective treatment, larvae were transferred to filtered water aquaria and assessed for alterations in endocrine-responsive gene expression (i.e., RT-qPCR), body size and survival. The remaining fish depurated in filtered water until reaching sexual maturity (180dph) for assessment of persistent effects on sex characteristics, reproductive performance and sex ratio. Following the 30-d early life exposure, larvae showed upregulations of the endocrine-responsive genes ar, erβ and vtg in response to both low and high doses of fulvestrant, but showed no differences in survival or body mass. Upon reaching sexual maturity under depuration conditions, male minnows previously exposed to fulvestrant as larvae showed reductions in gonad mass along with the feminization of secondary sex characteristics with no observed effects in females. Exposure to fulvestrant had no effects on gonadal histology, reproductive performance or final sex ratio as adults. Results from this study demonstrate that aqueous exposure to fulvestrant is estrogenic in fathead minnow larvae and is capable of feminizing male fish as adults following early life exposure. |
| Databáze: | OpenAIRE |
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