Liver-specific and high-level expression of human serum amyloid P component gene in transgenic mice
| Autor: | Takeaki Inomoto, Junichi Miyazaki, Ken Ichi Yamamura, Shuichiro Maeda, Shoji Wakasugi, Seiji Nishiguchi, Shuji Ohnishi, Tomohisa Iwanaga, Masashi Uno, Kimi Araki, Masahiro Uehira, Kazunori Shimada |
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| Rok vydání: | 1989 |
| Předmět: |
Lipopolysaccharides
Genetically modified mouse genetic structures Blotting Western Restriction Mapping Mice Transgenic Mice Gene expression Genetics Animals Humans RNA Messenger Gene Serum amyloid P component Inflammation Regulation of gene expression Messenger RNA Pentraxins biology Acute-phase protein Cell Biology Molecular biology eye diseases Blotting Southern Serum Amyloid P-Component Gene Expression Regulation biology.protein Injections Intraperitoneal Developmental Biology |
| Zdroj: | Developmental Genetics. 10:365-371 |
| ISSN: | 1520-6408 0192-253X |
| DOI: | 10.1002/dvg.1020100504 |
| Popis: | To analyze the regulation of human serum amyloid P component (SAP) gene expression, we have produced seven transgenic mice. The 3.3 kb human SAP genes containing about 0.8 kb of 5' and 1.5 kb of 3' flanking region were injected into fertilized eggs of C57BL/6 mice. In five of the seven transgenic mice, human SAP was detected in the sera and serum concentrations were higher than that of human serum in three lines. The human SAP gene was expressed only in the liver. Amounts of human mRNA in the liver and serum concentrations of human SAP were roughly proportional to the copy number of the integrated gene. Human SAP production lowered the serum levels of mouse endogenous SAP. With the intraperitoneal administration of lipopolysaccharide, the mRNA levels in the liver and serum levels of mouse SAP increased several-fold in both the control and transgenic mice. On the other hand, neither the mRNA nor the serum levels of human SAP increased significantly. |
| Databáze: | OpenAIRE |
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