Simulated biological fluid exposure changes nanoceria’s surface properties but not its biological response
| Autor: | Matthew L. Hancock, Marsha L. Ensor, Alexandra J. Brooks, Hemendra J. Vekaria, Eric A. Grulke, Patrick G. Sullivan, Benjamin Cherian, Robert A. Yokel |
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| Rok vydání: | 2019 |
| Předmět: |
Cell Survival
Surface Properties Cellular respiration Pharmaceutical Science chemistry.chemical_element 02 engineering and technology engineering.material medicine.disease_cause 030226 pharmacology & pharmacy Oxygen Article 03 medical and health sciences chemistry.chemical_compound Oxygen Consumption 0302 clinical medicine Coating Cell Line Tumor medicine Zeta potential Humans Biological activity Resazurin Cerium General Medicine 021001 nanoscience & nanotechnology Body Fluids Mitochondria Oxidative Stress chemistry A549 Cells engineering Biophysics Nanoparticles Caco-2 Cells 0210 nano-technology Citric acid Oxidation-Reduction Oxidative stress Biotechnology |
| Zdroj: | Eur J Pharm Biopharm |
| ISSN: | 0939-6411 |
| DOI: | 10.1016/j.ejpb.2019.09.023 |
| Popis: | Nanoscale cerium dioxide (nanoceria) has industrial applications, capitalizing on its catalytic, abrasive, and energy storage properties. It auto-catalytically cycles between Ce(3+) and Ce(4+), giving it pro-and anti-oxidative properties. The latter mediates beneficial effects in models of diseases that have oxidative stress/inflammation components. Engineered nanoparticles become coated after body fluid exposure, creating a corona, which can greatly influence their fate and effects. Very little has been reported about nanoceria surface changes and biological effects after pulmonary or gastrointestinal fluid exposure. The study objective was to address the hypothesis that simulated biological fluid (SBF) exposure changes nanoceria’s surface properties and biological activity. This was investigated by measuring the physicochemical properties of nanoceria with a citric acid coating (size; morphology; crystal structure; surface elemental composition, charge, and functional groups; and weight) before and after exposure to simulated lung, gastric, and intestinal fluids. SBF-exposed nanoceria biological effect was assessed as A549 or Caco-2 cell resazurin metabolism and mitochondrial oxygen consumption rate. SBF exposure resulted in loss or overcoating of nanoceria’s surface citrate, greater nanoceria agglomeration, deposition of some SBF components on nanoceria’s surface, and small changes in its zeta potential. The engineered nanoceria and SBF-exposed nanoceria produced no statistically significant changes in cell viability or cellular oxygen consumption rates. |
| Databáze: | OpenAIRE |
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