Development and trial of a dried tube specimen (DTS) proficiency testing panel for dual HIV/syphilis rapid diagnostic tests
| Autor: | Dumisile V. Maseko, Duduzile Valashiya, Ranmini Kularatne |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Pediatrics medicine.medical_specialty Laboratory Proficiency Testing Plasma samples business.industry Diagnostic Tests Routine Human immunodeficiency virus (HIV) Diagnostic test HIV Infections General Medicine Tube specimen Laboratory results medicine.disease medicine.disease_cause Syphilis Serodiagnosis Infectious Diseases Hiv test Proficiency testing medicine Humans Syphilis business |
| Zdroj: | Diagnostic microbiology and infectious disease. 102(3) |
| ISSN: | 1879-0070 |
| Popis: | Purpose To develop and trial a dried tube specimen (DTS) panel for proficiency testing (PT) of dual HIV/ syphilis rapid diagnostic tests (RDTs) at clinical sites. Results DTS panels were prepared using plasma samples with known HIV and syphilis results, to give varying reactivity for syphilis and HIV test lines on RDTs. Laboratory DTS panels were stable for a minimum 4-week period at ambient temperatures with no inter-reader variability of results. Field testing of panels with SD Bioline HIV/ Syphilis showed 100% correlation with laboratory results, and excellent mean pair agreement between the two clinical sites (k = 1.0). With Chembio DPP HIV/ Syphilis, there were two false negative results for HIV and syphilis, respectively, at one site; and good mean pair agreement between the two sites (k = 0.9). Conclusion It is feasible and practicable to incorporate DTS panels into a field PT scheme for dual HIV/ syphilis RDTs. |
| Databáze: | OpenAIRE |
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