ACE inhibitory activity in enzymatic hydrolysates of insect protein
| Autor: | Griet Herregods, Lieselot Vercruysse, Guy Smagghe, John Van Camp |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Proteases
Thermolysin Angiotensin-Converting Enzyme Inhibitors Grasshoppers Spodoptera Hydrolysate Lepidoptera genitalia Bombyx mori Animals Subtilisins Spodoptera littoralis Chromatography High Pressure Liquid chemistry.chemical_classification biology Hydrolysis fungi General Chemistry biology.organism_classification Bombyx Hymenoptera Enzyme Biochemistry chemistry Insect Proteins Schistocerca General Agricultural and Biological Sciences Peptide Hydrolases |
| Zdroj: | Journal of agricultural and food chemistry. 53(13) |
| ISSN: | 0021-8561 |
| Popis: | In this paper, ACE inhibitory activity in insect protein hydrolyzed by various enzymes (gastrointestinal proteases, alcalase, and thermolysin) is reported for the first time. Four insects of different insect orders were tested: Spodoptera littoralis (Lepidoptera), Bombyx mori (Lepidoptera), Schistocerca gregaria (Orthoptera), and Bombus terrestris (Hymenoptera). ACE inhibitory activity was measured by two different methods: a spectrophotometric method using FAPGG (2-furanacryloyl-phenylalanyl-glycyl-glycine) as substrate, and an HPLC method using dansyltriglycine (DTG) as substrate. Hydrolysis of the insect protein resulted in an increased ACE inhibitory activity. Overall, the highest ACE inhibitory activity was obtained after gastrointestinal digestion. These results suggest a role for insect protein as antihypertensive component in functional foods and nutraceuticals. Furthermore, the ACE inhibitory activity differed according to the method used. As a consequence, there is a need to standardize methodologies to evaluate ACE inhibitory activity. |
| Databáze: | OpenAIRE |
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