Sulfated polysaccharides of brown seaweeds - ligands of toll-like receptors
| Autor: | D. Yu. Logunov, N. N. Besednova, I. D. Makarenkova, Tatyana N. Zvyagintseva, A. I. Tukhvatulin, Svetlana P. Ermakova, V. I. Gorbach, I. B. Semenova |
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| Rok vydání: | 2012 |
| Předmět: |
Lipopolysaccharides
Chromatography Gas Lipopolysaccharide Sulfuric Acid Esters Biology Ligands General Biochemistry Genetics and Molecular Biology Cell Line chemistry.chemical_compound Sulfation Polysaccharides Transcription (biology) Humans Bioorganic chemistry Receptor Dose-Response Relationship Drug Fucoidan Toll-Like Receptors NF-kappa B Epithelial Cells General Medicine Seaweed Toll-Like Receptor 2 In vitro Toll-Like Receptor 4 Toll-Like Receptor 6 chemistry Biochemistry Cell culture Fucus Laminaria |
| Zdroj: | Biomeditsinskaya Khimiya. 58:318-325 |
| ISSN: | 2310-6905 2310-6972 |
| DOI: | 10.18097/pbmc20125803318 |
| Popis: | The interaction of sulfated polysaccharides - fucoidans from brown seaweeds Laminaria japonica, Laminaria cichorioides and Fucus evanescens with Toll-like receptors (TLRs) expressed on membranes of embryonic human kidney epithelial cells (HEK293-null, HEK293-TLR2/CD14, HEK293-hTLR4/CD14-MD2 and HEK293-hTLR2/6) was investigated. In vitro fucoidans specifically interacted with ТLR-2, ТLR-4, and the heterodimer ТLR-2/6 resultated in activation of transcription nuclear factor NF-κB. Analysis of composition the hydrolyzed fucoidan from F. evanescens was carried out by gas-liquid chromatography and chromatography-mass spectrometry. Results indicated the absence of 3-3-hydroxytetradecanoic acid (3-OHC14), the basic component of lipopolysaccharides in the preparation. Thus, the obtained results suggested that fucoidans from brown seaweeds possessing immunotropic activity are independent ligands for TLRs, and are able to induce genetically determined biochemical processes of protection organisms against pathogenic microorganisms. |
| Databáze: | OpenAIRE |
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