Development of a latex particles-based lateral flow immunoassay for group determination of macrolide antibiotics in breast milk
| Autor: | Anna Raysyan, Sergei A. Eremin, Maksim A. Burkin, Rudolf J. Schneider, Inna A. Galvidis |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Dirithromycin
medicine.drug_class Clinical Biochemistry Antibiotics Pharmaceutical Science Erythromycin Breast milk 01 natural sciences Analytical Chemistry Macrolide Antibiotics Clarithromycin Drug Discovery medicine Humans Spectroscopy Immunoassay Chromatography Oleandomycin Milk Human 010405 organic chemistry Chemistry Roxithromycin 010401 analytical chemistry Microspheres 0104 chemical sciences Anti-Bacterial Agents Macrolides medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 189 |
| ISSN: | 1873-264X |
| Popis: | A lateral flow immunoassay (LFIA) using latex particles labeled with antibody to BSA-clarithromycin (CLA) was developed for the rapid simultaneous group determination of six macrolide antibiotics. Optimization of antigen spotting on the membrane and latex probe loading allowed improving visual detectability (vLOD) 100 times, which was 1, 1, 10, 10, 50, and 1000 ng/mL for CLA, roxithromycin, erythromycin, dirithromycin, azithromycin, and oleandomycin in buffer, respectively. The calculated limits of instrumental detection (cLOD) were respectively 0.12, 0.15, 1.4, 2.1, 2.4, and 3.3 ng/mL. To avoid a strong influence of breast milk of a very diverse and variable composition, a sample pretreatment is proposed. The six macrolides mentioned can be visually detected in breast milk after 20 minutes pretreatment at concentrations of 10-1000 ng / ml or instrumentally with cLOD of 4.0, 2.5, 30, 42, 42 and 180 ng/mL. The recovery rate from the spiked samples carried out using a strip scanner device ranged from 71% to 110%, and precision expressed as relative standard deviation was between 3-14%. The described rapid on-site diagnostic assay format can be useful for monitoring the content of antibiotics in breast milk during macrolide treatment to ensure safe breastfeeding of infants. |
| Databáze: | OpenAIRE |
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