The use of a simple method to avoid cell shrinkage during SEM preparation
| Autor: | Uwe Gothe, Leo Wollweber, Roland Stracke |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Male
Histology Materials science Osmium Tetroxide Scanning electron microscope Cytological Techniques Cell Analytical chemistry Uranyl acetate Pathology and Forensic Medicine Mice chemistry.chemical_compound Tannic acid Critical point drying Organometallic Compounds medicine Animals Lymphocytes Shrinkage Macrophages Hydrolyzable Tannins medicine.anatomical_structure chemistry Glutaral Microscopy Electron Scanning Uranium Glutaraldehyde Nuclear chemistry |
| Zdroj: | Journal of Microscopy. 121:185-189 |
| ISSN: | 0022-2720 |
| DOI: | 10.1111/j.1365-2818.1981.tb01211.x |
| Popis: | SUMMARY In the course of preparing specimens for scanning electron microscopy, both glutaraldehyde and OsO4-fixed cells exhibit a considerable shrinkage with a reduction of the mean cellular diameter of about 45% after critical point drying. However, if cells are successively treated with glutaraldehyde, OsO4, tannic acid and uranyl acetate solutions, cellular shrinkage of only 5% is observed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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