Heterogeneity of Mouse Spleen Dendritic Cells: In Vivo Phagocytic Activity, Expression of Macrophage Markers, and Subpopulation Turnover
| Autor: | Pj, Leenen, Radosević K, Js, Voerman, Salomon B, van Rooijen N, David Klatzmann, van Ewijk W |
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| Přispěvatelé: | Immunology |
| Rok vydání: | 1998 |
| Předmět: |
Macrophages
Immunology Mice Transgenic Dendritic Cells Herpesvirus 1 Human Infusion Pumps Implantable CD13 Antigens Thymidine Kinase Mice Inbred C57BL Mice Phagocytosis SDG 3 - Good Health and Well-being Mice Inbred DBA Radiation Chimera Liposomes Animals Immunology and Allergy Clodronic Acid Ganciclovir Biomarkers Spleen HIV Long Terminal Repeat |
| Zdroj: | Europe PubMed Central Journal of Immunology, 160(5), 2166-2173. American Association of Immunologists Journal of Immunology, 160, 2166-2173. American Association of Immunologists |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | In the normal mouse spleen, two distinct populations of dendritic cells (DC) are present that differ in microanatomical location. The major population of marginal DC is found in the 'marginal zone bridging channels' and extends into the red pulp. The interdigitating cells (IDC) are localized in the T cell areas in the white pulp. The aim of the present study was to characterize these two splenic DC populations with regard to their phenotype, in vivo phagocytic function, and turnover. Both marginal DC and IDC are CD11c+ and CD13+, but only IDC are NLDC-145+ and CD8α+. Notably, both populations, when freshly isolated, express the macrophage markers F4/80, BM8, and Mac-1. To study the phagocytic capacity of these cells, we employed the macrophage 'suicide' technique by injecting liposomes loaded with clodronate i.v. Marginal DC, but not IDC, were eliminated by this treatment. Phagocytosis of DiI-labeled liposomes by DC confirmed this finding. The two DC populations differed significantly with regard to their turnover rates, as studied in a transgenic mouse model of conditional depletion of DC populations with high turnover. In these mice, marginal DC were completely eliminated, but the IDC population remained virtually intact. From these data we conclude that the marginal DC population has a high turnover, in contrast to the IDC population. Taken together, the present results indicate that marginal DC and IDC represent two essentially distinct populations of DC in the mouse spleen. They differ not only in location, but also in phenotype, phagocytic ability, and turnover. |
| Databáze: | OpenAIRE |
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