Quantification of main bioactive metabolites from saffron (Crocus sativus) stigmas by a micellar electrokinetic chromatographic (MEKC) method
| Autor: | Gyula Surányi, Sándor Gonda, Gábor Vasas, Gyöngyi Gyémánt, Péter Parizsa |
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| Rok vydání: | 2011 |
| Předmět: |
Clinical Biochemistry
Crocetin ved/biology.organism_classification_rank.species Pharmaceutical Science Disodium phosphate Electrolyte Analytical Chemistry law.invention chemistry.chemical_compound Electrolytes Természettudományok Glucosides law Limit of Detection Drug Discovery Crocus sativus Cyclohexenes Kémiai tudományok Spectroscopy Essential oil Micelles Chromatography Micellar Electrokinetic Capillary Detection limit Chromatography ved/biology Plant Extracts Terpenes Sodium Dodecyl Sulfate Hydrogen-Ion Concentration Crocus Picrocrocin Carotenoids Safranal chemistry Solvents |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 66 |
| ISSN: | 1873-264X |
| Popis: | Saffron is an expensive spice, cultivated in many regions of the world. Its chief metabolites include crocins, which are responsible for the coloring ability, safranal, which is the main essential oil constituent, and picrocrocin which is the main bitter constituent of the spice. A simple micellar capillary electrochromatographic (MEKC) method capable of quantifying all three types of main constituents was established. The pH, sodium dodecyl sulphate (SDS) content and electrolyte concentration of the background electrolyte was optimized. A simple extraction protocol was developed which can extract all metabolites of different polarity from the saffron stigmas. Optimal background electrolyte composed of 20 mM disodium phosphate, 5 mM sodium tetraborate, 100 mM SDS, pH was set 9.5. Optimal extracting solvent was the background electrolyte, incubated with the sample for 60 min. The proposed method allows quantification of picrocrocin, safranal, crocetin- Di-(β- d -gentiobiosyl) ester and crocetin (β- d -glycosyl)-(β- d -gentiobiosyl) ester within 17.5 min, with limit of detection values ranging from 0.006 to 0.04 mg/ml, from a single stigma. |
| Databáze: | OpenAIRE |
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