Interleukin-6 activates arginine vasopressin neurons in the supraoptic nucleus during immune challenge in rats
| Autor: | Karine Palin, Hélène Orcel, Julie Sauvant, Marie L. Moreau, Alain Rabié, Agnès Nadjar, Jennifer Dudit, Anne Duvoid-Guillou, Françoise Moos |
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| Přispěvatelé: | Psychoneuroimmunologie, nutrition et génétique, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
interleukine
Lipopolysaccharides Male Vasopressin prostaglandine Arginine Physiology Endocrinology Diabetes and Metabolism Interleukin-1beta Blood Pressure LIPOPOLYSACCHARIDE INDUCED-HYPOTHALAMIC VASOPRESSIN NEURON ACTIVATION BRAIN CYTOKINES DIURESIS PLASMA OSMOLALITY BLOOD PRESSURE RAT PRESSION ARTÉRIELLE ACTIVATION DES NEURONES Supraoptic nucleus Membrane Potentials 0302 clinical medicine neurone cytokine Neurons 0303 health sciences facteur de nécrose tumorale adn [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism medicine.anatomical_structure Hypothalamus Endocrinologie et métabolisme cerveau diurèse Supraoptic Nucleus hormones hormone substitutes and hormone antagonists noyau supraoptique medicine.medical_specialty Neuropeptide Biology Antibodies Dinoprostone 03 medical and health sciences Immune system pcr Physiology (medical) Internal medicine medicine Animals RNA Messenger Rats Wistar plasma 030304 developmental biology Inflammation Endocrinology and metabolism Interleukin-6 Tumor Necrosis Factor-alpha Osmolar Concentration osmolalité Electric Stimulation Diuresis Rats Plasma osmolality Arginine Vasopressin Endocrinology nervous system Neuron hormone antidiurétique 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology. Endocrinology and Metabolism 6 (296), E1289-E1299. (2009) AJP-Endocrinology and Metabolism AJP-Endocrinology and Metabolism, American Physiological Society, 2009, 296 (6), pp.E1289-E1299. ⟨10.1152/ajpendo.90489.2008⟩ |
| ISSN: | 0193-1849 1522-1555 |
| Popis: | The increase of plasma arginin-vasopressin (AVP) release, which translates hypothalamic AVP neuron activation in response to immune challenge, appears to occur independently of plasma osmolality or blood pressure changes. Many studies have shown that major inflammatory mediators produced in response to peripheral inflammation, such as prostaglandin (PG)-E2 and interleukin (IL)-1β, excite AVP neurons. However, in vivo electrical activation of AVP neurons was still not assessed in relation to plasma AVP release, osmolality, or blood pressure or to the expression and role of inflammatory molecules like PG-E2, IL-1β, IL-6, and tumor necrosis factor-α (TNFα). This study aims at elucidating those factors that underlie the activation of AVP neurons in response to immune stimulation mimicked by an intraperitoneal injection of lipopolysaccharide (LPS) in male Wistar rats. LPS treatment concomittanlty decreased diuresis and increased plasma AVP as well as AVP neuron activity in vivo, and these effects occurred as early as 30 min. Activation was sustained for more than 6 h. Plasma osmolality did not change, whereas blood pressure only transiently increased during the first hour post-LPS. PG-E2, IL-1β, and TNFα mRNA expression were raised 3 h after LPS, whereas IL-6 mRNA level increased 30 min post-LPS. In vivo electrophysiological recordings showed that brain IL-6 injection increased AVP neuron activity similarly to peripheral LPS treatment. In contrast, brain injection of anti-IL-6 antibodies prevented the LPS induced-activation of AVP neurons. Taken together, these results suggest that the early activation of AVP neurons in response to LPS injection is induced by brain IL-6. |
| Databáze: | OpenAIRE |
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