Suppression of cdc13-2-associated senescence by pif1-m2 requires Ku-mediated telomerase recruitment
| Autor: | Michael Chang, Fernando R. Rosas Bringas, Sonia Stinus, Enikő Fekete-Szücs |
|---|---|
| Přispěvatelé: | Damage and Repair in Cancer Development and Cancer Treatment (DARE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Senescence
AcademicSubjects/SCI01140 Telomerase Saccharomyces cerevisiae Proteins AcademicSubjects/SCI00010 Protein subunit Mutant Saccharomyces cerevisiae Telomere-Binding Proteins QH426-470 medicine.disease_cause AcademicSubjects/SCI01180 replicative senescence medicine Genetics Pif1 Molecular Biology Genetics (clinical) Investigation Mutation biology fungi DNA Helicases Helicase telomerase recruitment Telomere biology.organism_classification Cell biology Ku complex DNA-Binding Proteins Cdc13 biology.protein AcademicSubjects/SCI00960 |
| Zdroj: | bioRxiv. Cold Spring Harbor Labs Journals bioRxiv G3: Genes, Genomes, Genetics, Vol 12, Iss 1 (2021) G3: Genes|Genomes|Genetics G3 Genes|Genomes|Genetics, 12(1). GENETICS SOCIETY AMERICA |
| ISSN: | 2160-1836 |
| Popis: | In Saccharomyces cerevisiae, recruitment of telomerase to telomeres requires an interaction between Cdc13, which binds single-stranded telomeric DNA, and the Est1 subunit of telomerase. A second pathway involving an interaction between the yKu complex and telomerase RNA (TLC1) contributes to telomerase recruitment, but cannot sufficiently recruit telomerase on its own to prevent replicative senescence when the primary Cdc13-Est1 pathway is abolished—for example, in the cdc13-2 mutant. In this study, we find that mutation of PIF1, which encodes a helicase that inhibits telomerase, suppresses the replicative senescence of cdc13-2 increasing reliance on the yKu-TLC1 pathway for telomerase recruitment. Our findings reveal new insight into telomerase-mediated telomere maintenance. |
| Databáze: | OpenAIRE |
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