Further evidence for biological effects resulting from ionizing radiation doses in the diagnostic X-ray range
| Autor: | T. Conere, A McMahon, Shailendra Anoopkumar-Dukie, Ashley Allshire |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Radiobiology
Pilot Projects Pharmacology Nitric oxide Ionizing radiation HeLa chemistry.chemical_compound Radiation Ionizing Humans Medicine Radiology Nuclear Medicine and imaging Irradiation Radiation Injuries Cell Proliferation biology business.industry Cell growth X-Rays Dose-Response Relationship Radiation General Medicine biology.organism_classification Ascorbic acid Nitric oxide synthase chemistry biology.protein business Nuclear medicine HeLa Cells |
| Zdroj: | The British Journal of Radiology. 78:335-337 |
| ISSN: | 1748-880X 0007-1285 |
| DOI: | 10.1259/bjr/24426084 |
| Popis: | In this study we assessed cell response to ionizing radiation doses in the diagnostic X-ray range, using metabolic performance as the biological end point. HeLa cells were irradiated between 3.5 mGy (standard deviation (SD=2.5%) and 55.1 mGy (SD=0.9%) with 110 kV X-rays, and after 7 days metabolic capacity of cultures was measured spectrophotometrically with resazurin. Cells receiving 7.5 mGy or greater showed impaired proliferation. At 7.5 mGy this effect was prevented by nitric oxide synthase inhibitor N G-monomethyl-L-arginine but not by ascorbic acid or p38 MAPK inhibitor SB203580. We conclude that HeLa cell proliferation is adversely affected by doses within the range of some radiological examinations, through a mechanism involving nitric oxide. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|