Partial characterization of nif genes from the bacterium Azospirillum amazonense
| Autor: | Deise Porto Potrich, Irene Silveira Schrank, Luciane Maria Pereira Passaglia |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
glnB
Physiology Sequence analysis Operon Immunology Reação em cadeia da polimerase Molecular Sequence Data Biophysics Biology Biochemistry Genome Polymerase Chain Reaction Homology (biology) Upstream activating sequence 16S rDNA Nitrogen Fixation RNA Ribosomal 16S Amino Acid Sequence General Pharmacology Toxicology and Pharmaceutics Gene lcsh:QH301-705.5 Genomic organization Biologia molecular DNA Primers Genetics lcsh:R5-920 Base Sequence General Neuroscience Structural gene Gene Amplification nif genes Cell Biology General Medicine DNA Azospirillum amazonense Blotting Southern RNA Bacterial lcsh:Biology (General) Genes Bacterial RNA Hybridization Genetic Azospirillum lcsh:Medicine (General) Sequence Alignment Plasmids |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 34, Iss 9, Pp 1105-1113 (2001) Brazilian Journal of Medical and Biological Research v.34 n.9 2001 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Brazilian Journal of Medical and Biological Research, Volume: 34, Issue: 9, Pages: 1105-1113, Published: SEP 2001 |
| ISSN: | 0100-879X |
| Popis: | Azospirillum amazonense revealed genomic organization patterns of the nitrogen fixation genes similar to those of the distantly related species A. brasilense. Our work suggests that A. brasilense nifHDK, nifENX, fixABC operons and nifA and glnB genes may be structurally homologous to the counterpart genes of A. amazonense. This is the first analysis revealing homology between A. brasilense nif genes and the A. amazonense genome. Sequence analysis of PCR amplification products revealed similarities between the amino acid sequences of the highly conserved nifD and glnB genes of A. amazonense and related genes of A. brasilense and other bacteria. However, the A. amazonense non-coding regions (the upstream activator sequence region and the region between the nifH and nifD genes) differed from related regions of A. brasilense even in nitrogenase structural genes which are highly conserved among diazotrophic bacteria. The feasibility of the 16S ribosomal RNA gene-based PCR system for specific detection of A. amazonense was shown. Our results indicate that the PCR primers for 16S rDNA defined in this article are highly specific to A. amazonense and can distinguish this species from A. brasilense. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|