Could protein content of Urinary Extracellular Vesicles be useful to detect Cirrhosis in Alcoholic Liver Disease?
| Autor: | Janire Prieto-Elordui, Mikel Azkargorta, Esperanza Gonzalez, Felix Elortza, Clara Garcia-Vallicrosa, Sonia Blanco-Sampascual, Juan M. Falcón-Pérez |
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| Rok vydání: | 2021 |
| Předmět: |
Liver Cirrhosis
Male Proteomics medicine.medical_specialty Alcoholic liver disease Cirrhosis Urinary system Blotting Western Pilot Projects Urinalysis Applied Microbiology and Biotechnology Extracellular vesicles Gastroenterology Extracellular Vesicles Fibrosis Internal medicine medicine Humans Liquid biopsy Urinary Tract Liver Diseases Alcoholic Molecular Biology Ecology Evolution Behavior and Systematics alcoholic liver disease (ALD) liquid biopsy business.industry cirrhosis Cryoelectron Microscopy fibrosis Reproducibility of Results biomarkers Cell Biology Middle Aged medicine.disease Early Diagnosis Cohort business Research Paper urinary extracellular vesicles (uEVs) Developmental Biology |
| Zdroj: | International Journal of Biological Sciences |
| ISSN: | 1449-2288 |
| Popis: | Alcohol abuse has a high impact on the mortality and morbidity related to a great number of diseases and is responsible for the development of alcoholic liver disease (ALD). It remains challenging to detect and evaluate its severity, which is crucial for prognosis. In this work, we studied if urinary EVs (uEVs) could serve in diagnose and evaluate cirrhosis in ALD. To this purpose, uEVs characterization by cryo-electron microscopy (Cryo-EM), Nanoparticle Tracking Analysis (NTA) and Western blotting (WB) was performed in a cohort of 21 controls and 21 cirrhotic patients. Then, proteomics of uEVs was carried out in a second cohort of 6 controls and 8 patients in order to identify new putative biomarkers for cirrhosis in ALD. Interestingly, uEVs concentration, size and protein composition were altered in cirrhotic patients. From a total of 1304 proteins identified in uEVs, 90 of them were found to be altered in cirrhotic patients. The results suggest that uEVs could be considered as a tool and a supplier of new biomarkers for cirrhosis in ALD, whose application would be especially relevant in chronic patients. Yet, further research is necessary to obtain more relevant result in clinical terms. |
| Databáze: | OpenAIRE |
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