Development of highly sensitive quantification method for testosterone and dihydrotestosterone in human serum and prostate tissue by liquid chromatography–electrospray ionization tandem mass spectrometry
| Autor: | Mitsunobu Okuyama, Kouwa Yamashita, Madoka Takahashi, Yoshimichi Miyashiro, Hitoe Maekubo, Mitsuteru Numazawa, Seijiro Honma |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Spectrometry Mass Electrospray Ionization Time Factors Adolescent medicine.drug_class Clinical Biochemistry Picolinic acid Mass spectrometry Tandem mass spectrometry Biochemistry Specimen Handling Young Adult chemistry.chemical_compound Endocrinology Tandem Mass Spectrometry Prostate medicine Humans Testosterone Picolinic Acids Derivatization Molecular Biology Aged Aged 80 and over Pharmacology Chromatography Organic Chemistry Dihydrotestosterone Esters Middle Aged Androgen medicine.anatomical_structure chemistry Calibration Linear Models Female Blood Chemical Analysis Chromatography Liquid medicine.drug |
| Zdroj: | Steroids. 74:920-926 |
| ISSN: | 0039-128X |
| DOI: | 10.1016/j.steroids.2009.06.007 |
| Popis: | We developed highly sensitive detection of testosterone (T) and 5α-dihydrotestosterone (DHT) by liquid chromatography–electrospray ionization tandem mass spectrometry using high proton affinitive derivatization of 17β-hydroxyl group of T and DHT with picolinic acid, mobile phase consisting of MeCN–MeOH–H2O–formic acid and conventional octadecylsilica (ODS) column. Purification of the derivatives was carried out using solid-phase extraction with ODS cartridge. By this method, T and DHT were determined simultaneously with limits of quantification (LOQs) of 1 pg/0.2 ml in serum, and T and DHT with LOQs of 0.5 pg and 1 pg/3 mg in prostate tissue, respectively, under acceptable assay performance (intra-assay and inter-assay accuracy and precision). The present method provides reliable and reproducible results for quantification of T and DHT in small volumes of serum and prostate samples for diagnosis in prostatic disorders and male climacteric. |
| Databáze: | OpenAIRE |
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