Protective effect of Withania somnifera and Cardiospermum halicacabum extracts against collagenolytic degradation of collagen
| Autor: | P.K. Sehgal, Krishnamoorthy Ganesan, Sadulla Sayeed, Asit Baran Mandal |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Bioengineering
Withania somnifera Matrix metalloproteinase Withania Cardiospermum halicacabum Applied Microbiology and Biotechnology Biochemistry Collagen Type I Protein Structure Secondary Extracellular matrix Arthritis Rheumatoid Hydroxyproline chemistry.chemical_compound Sapindaceae Osteoarthritis medicine Animals Humans Protease Inhibitors Molecular Biology integumentary system biology Dose-Response Relationship Drug Chemistry Plant Extracts Cartilage Circular Dichroism Hydrolysis General Medicine biology.organism_classification Molecular biology Extracellular Matrix Solutions Kinetics medicine.anatomical_structure Microbial Collagenase Collagenase Cattle Type I collagen Biotechnology medicine.drug |
| Zdroj: | Applied biochemistry and biotechnology. 165(3-4) |
| ISSN: | 1559-0291 |
| Popis: | The irreversible destruction of extracellular matrix (ECM) such as cartilage, tendon, and bone that comprise synovial joints is the hallmark of both rheumatoid arthritis and osteoarthritis by over-expression of matrix metalloproteinase (MMP)-collagenases. We report herein the detailed study on the inhibitory effects of Withania somnifera extract (WSE) and Cardiospermum halicacabum extract (CHE) on Clostridium histolyticum collagenase (ChC) activity against the degradation of the ECM component of bovine Achilles tendon type I collagen by hydroxyproline assay method. Interaction of WSE and CHE with ChC exhibited 71% and 88% inhibition, respectively, to the collagenolytic activity of ChC against collagen degradation, and the inhibition was found to be concentration-dependent. The inhibition kinetics of ChC by both the extracts has been deduced from the extent of hydrolysis of N-[3-(2-furyl) acryloyl]-Leu-Gly-Pro-Ala. Both WSE and CHE are provided competitive and mixed type inhibition on ChC activity, respectively. Circular dichroism studies of ChC on treatment with WSE and CHE revealed changes in the secondary structure of collagenase. These results suggest that the WSE and CHE facilitated collagen stabilization through collagenase inhibition. |
| Databáze: | OpenAIRE |
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