Expression profile of lncRNAs and mRNAs in intestinal macrophages
| Autor: | Qais Ahmad Naseer, Jianguo Qu, Xiaogang Wang, Lu-Lu Liu, Jixiang Chen, Xiaofei Xue, Lei Cui, Sheng-Chun Dang, Siche Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine Cancer Research Lipopolysaccharide mRNA Cell Biology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Downregulation and upregulation Genetics medicine Animals Gene Regulatory Networks RNA Messenger Molecular Biology Cells Cultured Oligonucleotide Array Sequence Analysis long non-coding RNA Oncogene Microarray analysis techniques Gene Expression Profiling Macrophages Articles intestinal macrophages Cell cycle Rats Cell biology Intestines Gene Ontology 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Oncology chemistry Apoptosis 030220 oncology & carcinogenesis Molecular Medicine Female RNA Long Noncoding Signal transduction expression profiles |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2020.11470 |
| Popis: | Non-coding RNAs (ncRNAs) have been previously reported to serve an important role in transcription. In addition, several studies have revealed that long ncRNAs (lncRNAs) have a crucial role in human diseases. However, the association between lncRNAs and inflammation-induced intestinal macrophages in the intestinal mucosal barrier has remained elusive. In the present study, intestinal macrophages from healthy Sprague Dawley rats were divided into two groups: The experimental group, consisting of intestinal macrophages treated with 1 mg/l lipopolysaccharide (LPS) and the control group, composed of untreated cells. Differentially expressed (DE) lncRNAs and mRNAs between the control and experimental groups were identified using microarray profiling. The levels of DE mRNAs and lncRNAs were measured by reverse transcription-quantitative PCR (RT-qPCR). Furthermore, Gene Ontology (GO) and pathway enrichment analyses of DE mRNAs and lncRNAs were performed. To identify core regulatory factors among DE lncRNAs and mRNAs, a lncRNA-mRNA network was constructed. A total of 357 DE lncRNAs and 542 DE mRNAs between the LPS-treated and untreated groups were identified (fold-change >1.5; P |
| Databáze: | OpenAIRE |
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