Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases
| Autor: | Shuyuan Jiang, You Liu, Xiaoli Tian, Ming Zhang, Guo Shao, He Wang, Rui Shi, Zhan-Jun Yang, Shengdi Xu, Shaochun Yan, Xiaolu Zhang, Xiaolei Liu, Hong-Xia Zheng, Kerui Gong |
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| Rok vydání: | 2018 |
| Předmět: |
Neurons
0301 basic medicine Methyltransferase Health Toxicology and Mutagenesis Cellular differentiation Cell Differentiation Biology Toxicology Hippocampus DNA methyltransferase Neural stem cell Cell Line Cell biology Mice 03 medical and health sciences 030104 developmental biology Nerve growth factor Cell culture Nerve Growth Factor DNA methylation Animals DNA (Cytosine-5-)-Methyltransferases Epigenetics |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 81:1116-1122 |
| ISSN: | 1087-2620 1528-7394 |
| DOI: | 10.1080/15287394.2018.1504384 |
| Popis: | DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway. |
| Databáze: | OpenAIRE |
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