Expression of Th1 and Th2 immunoregulatory cytokines by human eosinophils
| Autor: | Sylvie Loiseau, Monique Capron, Gaëtane Woerly, Nadine Roger |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chemokine
biology medicine.medical_treatment Immunology Inflammation General Medicine Eosinophil Th1 Cells Proinflammatory cytokine Eosinophils Cytokine medicine.anatomical_structure Immune system Th2 Cells medicine biology.protein Immunology and Allergy Cytotoxic T cell Animals Cytokines Humans medicine.symptom Intracellular |
| Zdroj: | International archives of allergy and immunology. 118(2-4) |
| ISSN: | 1018-2438 |
| Popis: | Besides cytotoxic mediators, human eosinophils can produce proinflammatory and anti–inflammatory cytokines, as well as growth factors and chemokines. The demonstration that eosinophils from patients could produce IL–5, IL–4 and IL–2 suggested their participation in the regulation of immune response. In the present work, we have examined the presence of Th1 (IFN–γ, IL–2) and Th2 (IL–4, IL–5, IL–10 and IL–13) cytokines in eosinophils from patients or donors by intracellular flow cytometry, and by immunocytochemistry. Whereas almost 100% eosinophils expressed intracellular IFN–γ, IL–2 and IL–10, the expression of IL–4, IL–5 and IL–13 is more variable and increased in patients versus donors. The differential release by eosinophils of Th2 versus Th1 cytokines is suggested both by the decrease of the intracellular content after culture, restricted to IL–4, IL–5 and IL–13, associated with an accumulation of IL–4 and IL–5 in the presence of Brefeldin. These results indicate that, through the preferential release of Th2 cytokines, human eosinophils can participate in the polarization of the immune response. |
| Databáze: | OpenAIRE |
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