Molecular epidemiology of photobacterium damselae subsp. damselae outbreaks in marine rainbow trout farms reveals extensive horizontal gene transfer and high genetic diversity
| Autor: | Carlos R. Osorio, Karl Pedersen, Xosé M. Matanza, Mateus S. Terceti, Inger Dalsgaard, Ana Vences |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía, Universidade de Santiago de Compostela. Instituto de Acuicultura |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Fish farming animal diseases Hemolysin lcsh:QR1-502 Virulence Zoology Biology Microbiology lcsh:Microbiology Damselysin 03 medical and health sciences SDG 14 - Life Below Water phobalysin Original Research Genetic diversity Molecular epidemiology Photobacterium damselae vibriosis rainbow trout Phobalysin hemolysin 030104 developmental biology Rainbow trout Horizontal gene transfer damselysin Vibriosis Mobile genetic elements |
| Zdroj: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Frontiers in Microbiology Terceti, M S, Vences, A, Matanza, X M, Dalsgaard, I, Pedersen, K & Osorio, C R 2018, ' Molecular epidemiology of Photobacterium damselae subsp damselae outbreaks in marine rainbow trout farms reveals extensive horizontal gene transfer and high genetic diversity ', Frontiers in Microbiology, vol. 9, 2155 . https://doi.org/10.3389/fmicb.2018.02155 Frontiers in Microbiology, Vol 9 (2018) |
| DOI: | 10.3389/fmicb.2018.02155 |
| Popis: | The marine bacterium Photobacterium damselae subsp. damselae is a pathogen for a variety of marine animals, as well as for humans, and is nowadays considered an emerging pathogen for fish of importance in marine aquaculture. Recent studies have suggested that outbreaks in fish farms are caused by multiclonal populations of this subspecies that exist in the environment. Here, we report the study of a collection of 31 strains isolated during the course of disease outbreaks in marine rainbow trout farms in Denmark in 1994, 1995, and 2006, respectively. A phylogenetic analysis based on the toxR gene sequence, and the screening of virulence-related genes uncovered a high genetic heterogeneity, even among strains isolated from the same fish farm at the same time. Moreover, comparative analysis of the whole genome sequences of four selected strains revealed a large number of differentially occurring genes, which included virulence genes, pPHDD1 plasmid, polysaccharide synthesis gene clusters, CRISPR-Cas systems and putative new mobile genetic elements. This study provides sound evidence that P. damselae subsp. damselae outbreaks in Danish rainbow trout farms were caused by multiclonal populations and that horizontal gene transfer constitutes a strong driving force in the generation of intraspecific diversity in this pathogen This work has been supported by grant AGL2016-79738-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, and co-funded by the FEDER Programme from the European Union. The support of Xunta de Galicia (Spain) with grant GRC-2014/007 is also acknowledged. MT thanks the Brazilian Ministry of Education and CAPES (Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior) for a predoctoral fellowship. XM thanks Xunta de Galicia for a predoctoral fellowship SI |
| Databáze: | OpenAIRE |
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