Comparison of two commercial embryo culture media (SAGE-1 step single medium vs. G1-PLUSTM/G2-PLUSTM sequential media): Influence on in vitro fertilization outcomes and human embryo quality
| Autor: | Pablo Javier Moreno de Acevedo-Yagüe, Ana Isabel Mangano Armada, Javier María Gutiérrez-Romero, María Mercedes Calero-Ruiz, Iratxe López-Pelayo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
human embryo quality Pregnancy Rate medicine.medical_treatment Embryonic Development Biology Miscarriage Embryo Culture Techniques Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization Embryo cryopreservation Pregnancy medicine Humans Retrospective Studies 030219 obstetrics & reproductive medicine In vitro fertilisation pregnancy rates SAGE Pregnancy Outcome human embryo development Embryo culture Embryo frozen embryos medicine.disease Culture Media 030104 developmental biology Fertilization Culture media system Female Original Article Embryo quality |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 |
| Popis: | OBJECTIVE To compare embryo quality, fertilization, implantation, miscarriage and clinical pregnancy rates for embryos cultured in two different commercial culture media until D-2 or D-3. METHODS In this retrospective study, we analyzed 189 cycles performed in 2016. Metaphase II oocytes were microinjected and allocated into single medium (SAGE 1-STEP, Origio) until transferred, frozen or discarded; or, if sequential media were used, the oocytes were cultured in G1-PLUSTM (Vitrolife) up to D-2 or D-3 and in G2-PLUSTM (Vitrolife) to transfer. On the following day, the oocytes were checked for normal fertilization and on D-2 and D-3 for morphological classification. Statistical analysis was performed using the chi-square and Mann-Whitney tests in PASW Statistics 18.0. RESULTS The fertilization rates were 70.07% for single and 69.11% for sequential media (p=0.736). The mean number of embryos with high morphological quality (class A/B) was higher in the single medium than in the sequential media: D-2 [class A (190 vs. 107, p |
| Databáze: | OpenAIRE |
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