Outer-membrane protein and immunoblot analysis of Australian isolates of Haemophilus influenzae
| Autor: | A. Lawrence, D. Hansman |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Microbiology (medical)
Serotype Haemophilus Infections Blotting Western medicine.disease_cause Microbiology Haemophilus influenzae Immunoblot Analysis medicine Humans Typing Child biology Pasteurellaceae Australia General Medicine biology.organism_classification medicine.disease Virology Subtyping Bacterial Typing Techniques Electrophoresis Polyacrylamide Gel Bacterial outer membrane Meningitis Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of Medical Microbiology. 38:378-383 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-38-5-378 |
| Popis: | Summary Strains of Haemophilus influenzae isolated from children in South Australia and the Northern Territory with systemic infections (mostly meningitis or epiglottitis) were subjected to serotyping, biotyping, outer-membrane protein (OMP) analysis and immunoblot subtyping. All 65 isolates examined were from blood or cerebrospinal fluid; 59 (91%) of the strains were identified as type b and the remainder as either type a (two strains) or non-typable (four strains). Of the 59 type b strains, 45 (76%) belonged to a single OMP subtype (equivalent to subtype 3L in the Barenkamp scheme); the remaining type b strains belonged to five other OMP subtypes. No correlation was apparent between OMP subtype and geographical region, clinical diagnosis or antimicrobial drug susceptibility pattern. Immunoblot subtyping enabled nine (18%) of 41 strains belonging to the principal OMP subtype to be distinguished from the remainder. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|