Linear Multiepitope (Glyco)peptides for Type-Specific Serology of Herpes Simplex Virus (HSV) Infections
| Autor: | Ola Blixt, Tomas Bergström, Kasper K. Sørensen, Sigvard Olofsson, Christian Risinger, Knud J. Jensen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
viruses Herpesvirus 2 Human Protein Array Analysis Antigen-Antibody Complex Herpesvirus 1 Human medicine.disease_cause Antibodies Viral Immunoglobulin G Epitope Serology Diagnosis Differential 03 medical and health sciences Epitopes 0302 clinical medicine Species Specificity medicine Humans 030212 general & internal medicine Microwaves Antigens Viral Glycoproteins chemistry.chemical_classification Herpes Genitalis biology Immune Sera Herpes Simplex Virology Glycopeptide 3. Good health 030104 developmental biology Infectious Diseases Epitope mapping Herpes simplex virus chemistry biology.protein Antibody Glycoprotein Epitope Mapping |
| Zdroj: | ACS Infectious Diseases |
| ISSN: | 2373-8227 |
| Popis: | Detection of type-specific antibodies is an important and essential part of accurate diagnosis, even in silent carriers of herpes simplex virus (HSV)-1 (oral) and HSV-2 (genital) infections. Serologic assays that identify HSV-1 and HSV-2 type-specific antibodies have been commercially available for more than a decade but often face problems related to cross-reactivity and similar issues. Attempts to identify type-specific peptide epitopes for use in serology for both HSV-1 and HSV-2 have been limited. We recently demonstrated epitope mapping of envelope glycoprotein G2 and identified a type-specific glycopeptide epitope that broadly recognized HSV-2 infected individuals. In the present work we have performed a comprehensive glycopeptide synthesis and microarray epitope mapping of 14 envelope proteins from HSV-1 and HSV-2, namely, gB, gC, gD, gE, gG, gH, and gI, using sera from HSV-1- and HSV-2-infected individuals and control sera. Several unique type-specific peptide epitopes with high sensitivity were identified and synthesized as one large linear multiepitope sequence using microwave-assisted solid-phase (glyco)peptide synthesis. Microarray validation with clinically defined HSV and Varicella Zoster (VZV) sera confirmed excellent cumulative specificities and sensitivities. |
| Databáze: | OpenAIRE |
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