SRPP, a cell-wall protein is involved in development and protection of seeds and root hairs in Arabidopsis thaliana
| Autor: | Ali Ferjani, Hiroshi Uno, Shizuka Gunji, Natsuki Tanaka, Takashi Aoyama, Masayoshi Maeshima, Shohei Okuda |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death Physiology Green Fluorescent Proteins Mutant Arabidopsis Flowers Plant Science Root hair Biology Plant Roots 01 natural sciences Phosphates Green fluorescent protein Cell wall Gene Knockout Techniques 03 medical and health sciences chemistry.chemical_compound Cell Wall Gene Expression Regulation Plant Botany Arabidopsis thaliana Propidium iodide integumentary system Arabidopsis Proteins Embryo Cell Biology General Medicine Ethylenes Plants Genetically Modified biology.organism_classification Cell biology Microscopy Electron 030104 developmental biology chemistry Fruit Seeds sense organs 010606 plant biology & botany |
| Zdroj: | Plant and Cell Physiology. :pcx008 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcx008 |
| Popis: | Enhancement of root hair development in response to phosphate (Pi) deficit has been reported extensively. Root hairs are involved in major root functions such as the absorption of water, acquisition of nutrients and secretion of organic acids and enzymes. Individual root hair cells maintain these functions and appropriate structure under various physiological conditions. We carried out a study to identify protein(s) which maintain the structure and function of root hairs, and identified a protein (SEED AND ROOT HAIR PROTECTIVE PROTEIN, SRPP) that was induced in root hairs under Pi-deficient conditions. Promoter assay and mRNA quantification revealed that SRPP was expressed in root hairs and seeds. A knockout mutant, srpp-1, consistently displayed defects in root hairs and seeds. Root hairs in srpp-1 were short and the phenotypes observed under Pi-deficient conditions were also detected in ethylene-treated srpp-1 plants. Propidium iodide stained most root hairs of srpp-1 grown under Pi-deficient conditions, suggesting cell death. In addition to root hairs, most srpp-1 seeds were withered and their embryos were dead. SRPP tagged with green fluorescent protein was detected in the cell wall. Electron microscopy showed abnormal morphology of the cell wall. Wild-type phenotypes were restored when the SRPP gene was expressed in srpp-1. These data strongly suggest that SRPP contributes to the construction of robust cell walls, whereby it plays a key role in the development of root hairs and seeds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|