Validation of Housekeeping Genes as Internal Controls for the Study of the Effects of Microcystin-LR in Zebrafish by Real-Time PCR

Autor:	Mauricio da Silva Sopezki, José Maria Monserrat, João Sarkis Yunes, Juliano Zanette
Rok vydání:	2018
Předmět:	0301 basic medicine Arginine Microcystins Microcystin-LR 010501 environmental sciences Biology medicine.disease_cause Real-Time Polymerase Chain Reaction 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene expression polycyclic compounds medicine Animals Enzyme Inhibitors Gene Zebrafish 0105 earth and related environmental sciences Genes Essential Toxin Reference Standards biology.organism_classification Molecular biology Housekeeping gene 030104 developmental biology Real-time polymerase chain reaction chemistry Gene Expression Regulation Liver Animal Science and Zoology Marine Toxins Developmental Biology
Zdroj:	Zebrafish. 15(5)
ISSN:	1557-8542
Popis:	Microcystin-leucine arginine (MC-LR) is a natural toxin produced by cyanobacterial blooms that causes severe liver damage in fish. It is crucial to investigate if housekeeping genes are affected by MC-LR in zebrafish, to permit the adequate evaluation of gene expression by RT-qPCR. We evaluated the gene expression stability (M value) and regulation by chemical treatment (using E
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6afdf75fe0bb7f66cabf0ed77167a6b https://pubmed.ncbi.nlm.nih.gov/30044200 Zobrazit plný text záznamu