Anti-inflammatory effects of conjugated linoleic acid isomers and essential fatty acids in bovine mammary epithelial cells
| Autor: | Umberto Bernabucci, A. Tröscher, L. Basiricò, D. Dipasquale, P. Morera, Riccardo Primi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
CLA cis-9 Lipopolysaccharide medicine.drug_class Conjugated linoleic acid Linoleic acid Inflammation essential fatty acids medicine.disease_cause SF1-1100 Anti-inflammatory Proinflammatory cytokine Linoleic Acid bovine mammary gland cells 03 medical and health sciences chemistry.chemical_compound Mammary Glands Animal Isomerism CLA trans-10 CLA cis-9 trans-11 CLA trans-10 cis-12 essential fatty acids bovine mammary gland cells inflammatory status medicine Animals Linoleic Acids Conjugated Fatty Acids trans-11 0402 animal and dairy science Epithelial Cells cis-12 04 agricultural and veterinary sciences 040201 dairy & animal science Molecular biology Animal culture 030104 developmental biology chemistry Cytokines Cattle Animal Science and Zoology Tumor necrosis factor alpha lipids (amino acids peptides and proteins) medicine.symptom Oxidative stress |
| Zdroj: | Animal, Vol 12, Iss 10, Pp 2108-2114 (2018) |
| ISSN: | 1751-7311 |
| DOI: | 10.5281/zenodo.3828454 |
| Popis: | Anti-inflammatory effects of conjugated linoleic acid isomers and essential fatty acids in bovine mammary epithelial cells D. Dipasquale1, L. Basiricò1, P. Morera1, R. Primi1, A. Tröscher2 and U. Bernabucci1† 1Dipartimento di Scienze Agrarie e Forestali, Università degli Studi della Tuscia, via San Camillo De Lellis s.n.c., 01100 Viterbo, Italy; 2Animal Nutrition, BASF SE,Chemiestrasse. 22, F 31, 68623 Lampertheim, Germany Animal, vol. 9, p..1 -7. doi:10.1017/S1751731117003676 Fatty acids are important modulators of inflammatory responses, in particular, n-3 and n-6 essential fatty acids and CLA have received particular attention for their ability to modulate inflammation. The objectives of this study were to compare the effects of CLA and essential fatty acids on the expression of pro and anti- inflammatory cytokines and their protective efficacy against inflammatory status in mammary gland by an in vitro model based on bovine mammary epithelial cells (BME-UV1). Bovine mammary epithelial cells were treated with complete medium containing either 50 μM of cis-9, trans-11 CLA (c9,t11 CLA) or trans-10, cis-12 CLA (t10,c12 CLA) or (α)-linolenic acid (aLnA) or (γ)-linolenic acid (gLnA) or linoleic acid (LA). After 48 h by fatty acids administration the cells were treated for 3 h with 20 μM of lipopolysaccharide (LPS) to induce inflammatory stimulus. Reactive oxygen species (ROS) production after treatments was assessed to verify and to compare the potential protection of different fatty acids against LPS-induced oxidative stress. The messenger RNA abundance of bovine pro and anti-inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β),interleukin-6 (IL-6) and interleukine-10 (IL-10)) and peroxisome proliferator receptor-α/γ (PPARγ/α) were determined in BME-UV1 by realtime PCR. The results showed that cells treated with fatty acids and LPS increased ROS production compared with control cells. Among treatments, cells treated with c9,t11 CLA and t10,c12 CLA isomers revealed significant lower levels of ROS production compared with other fatty acids. All fatty acids reduced the gene expression of pro- and anti-inflammatory cytokines. Among fatty acids, t10,c12 CLA, LA and gLnA showed an homogeneous reduction of the three pro-inflammatory cytokines and this may correspond to more balanced and efficient physiological activity and may trigger a better protective effect. The PPARγ gene expression was significantly greater in cells treated with t10,c12 CLA, aLnA and LA, whereas the PPARα gene expression levels were significantly lower in cells treated with all different fatty acids, compared with the control. These results suggest that fatty acids inhibited the transcription of pro-inflammatory cytokines by the upregulation of PPARγ expression. The supplementation of CLA and essential fatty acids (EFAs) in bovine mammary epithelial cell line exposed to severe inflammatory conditions, reduced the gene expression of proinflammatory cytokines. This study suggests and strengthen the utilization of these fatty acids as a useful management tool for dairy cattle, their use on high-yielding dairy cow at the time of calving, could act by reducing the development of inflammatory processes related to parturition. |
| Databáze: | OpenAIRE |
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