Reconstruction of Penile Urethra With the 3-Dimensional Porous Bladder Acellular Matrix in a Rabbit Model
| Autor: | Yuemin Xu, Chao Li, Yuanyuan Zhang, Hong-Bin Li, Gai-Jie Wei, Lujie Song, Jihong Wang, Xiang Li, Jianwen Huang, Minkai Xie, Wei-Dong Zhu |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
Acellular Dermis medicine.medical_specialty Urology Urethroplasty medicine.medical_treatment Fistula Sensitivity and Specificity Random Allocation Imaging Three-Dimensional Urethra Tissue engineering medicine Animals Urothelium Analysis of Variance Tissue Engineering medicine.diagnostic_test business.industry Biopsy Needle food and beverages Anatomy Plastic Surgery Procedures biochemical phenomena metabolism and nutrition medicine.disease Immunohistochemistry Disease Models Animal medicine.anatomical_structure Retrograde urethrogram Rabbit model Rabbits business Penis |
| Zdroj: | Urology. 84:1499-1505 |
| ISSN: | 0090-4295 |
| DOI: | 10.1016/j.urology.2014.07.044 |
| Popis: | To evaluate the effect of reconstruction of penile urethra with the 3-dimensional (3-D) porous bladder acellular matrix (BAM) in a rabbit model.In 30 male rabbits, a ventral urethral mucosal defect (1.5 × 0.8 cm) was created. Substitution urethroplasty was performed with 5% peracetic acid (PAA)-treated BAM (3-D porous BAM; 15 rabbits, PAA-treated BAM group) and non-PAA-treated BAM (15 rabbits; non-PAA-treated BAM group) in an onlay fashion. At 1, 2, and 3 months after surgery (5 rabbits at each time point) in the 2 groups, retrograde urethrogram and histologic analysis were performed to evaluate the outcomes of urethroplasty.In the PAA-treated BAM group, 13 rabbits maintained a wide urethral caliber without a fistula or stricture. In contrast, 10 rabbits kept a wide caliber in the non-PAA-treated BAM group. Histologically, at 1, 2, and 3 months after the surgery, the speed of urothelium regeneration in the PAA-treated BAM group was faster than that in the non-PAA-treated BAM group. The smooth muscle-to-collagen ratio and the content of smooth muscle in the PAA-treated BAM group were significantly higher than that in the non-PAA-treated BAM group at each time point (P.05). The endothelium density between the non-PAA-treated BAM and the PAA-treated BAM groups revealed a significant increase at all 3 time points (P.05).Our results confirmed that PAA-treated BAM urethroplasty enhanced urothelium, smooth muscle regeneration and neovascularization compared with those of the non-PAA-treated BAM. The 3-D porous BAM as an optimized biological scaffold may be used for cell-based tubular and long-segmental urethral reconstruction in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect