The effects of commercially available preservative-free FDA-approved triamcinolone (Triesence®) on retinal cells in culture
| Autor: | Baruch D. Kuppermann, Maria C. Kenney, Claudio Ramirez, Leandro Cabral Zacharias, Gail M. Seigel, Walter Yukihiko Takahashi, M. F. Estrago-Franco |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Preservative
medicine.medical_specialty Triamcinolone acetonide medicine.drug_class Cell Survival Apoptosis Triamcinolone Retina chemistry.chemical_compound Internal medicine medicine Animals Humans Pharmacology (medical) Viability assay Cells Cultured Pharmacology Caspase 7 Chromatography Dose-Response Relationship Drug Dimethyl sulfoxide business.industry Caspase 3 Retinal Rats Ophthalmology Endocrinology chemistry Cell culture Corticosteroid Trypan blue business medicine.drug |
| Zdroj: | Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics. 27(2) |
| ISSN: | 1557-7732 |
| Popis: | To evaluate the effects of Triesence® (TRI), a new preservative-free triamcinolone approved by the U.S. Food and Drug Administration (FDA) for intraocular use, on human retina pigment epithelial (ARPE-19) and rat neurosensory (R28) cells in culture.ARPE-19 and R28 cell cultures were treated 24 h with 1,000, 500, 200, or 100 μg/mL of crystalline (cTRI) or 1,000, 500, or 200 μg/mL of solubilized (sTRI). TRI was solubilized by centrifuging the drug, discarding the supernatant containing the vehicle and then resuspending the drug pellet in an equivalent amount of Dimethyl sulfoxide to achieve the same concentration as the commercial preparation. Percentage of cell viability (CV) was evaluated by a trypan blue dye-exclusion assay. The mitochondrial membrane potential (ΔΨm) was analyzed with the JC-1 assay. The caspase-3/7 activity was measured by a fluorochrome assay.In the ARPE-19 cultures, the cTRI caused a decrease in CV at 1,000 μg/mL (13.03±6.51; P0.001), 500 μg/mL (28.87±9.3; P0.001), 200 μg/mL (54.93±5.61; P0.001), and 100 μg/mL (82.53±0.65; P0.005) compared with the untreated controls (96.98±0.16). In R28 cultures, the cTRI treatment also reduced CV values significantly (P0.001) for the 1,000 μg/mL (22.73±2.44), 500 μg/mL (34.63±1.91), 200 μg/mL (58.70±1.39), and 100 μg/m (75.33±2.47) compared with the untreated controls (86.08±3.54). Once the TRI was solubilized (sTRI), the CV and ΔΨm remained similar to the untreated controls for both ARPE-19 and R28 cells. The sTRI treatment with 1,000, 500, and 200 μg/mL increased in caspase-3/7 activity in ARPE-19 cells (P0.01) and in R28 cells (P0.05) compared with dimethyl sulfoxide equivalent controls.The crystalline form of TRI (cTRI) can cause a significant decrease in CV to cultured retinal cells. Once the TRI is solubilized (sTRI), at the same concentrations, the cells remain viable with no decrease in CV or ΔΨm. The sTRI can, however, increase caspase-3/7 activity, thus suggesting some degree of apoptosis. |
| Databáze: | OpenAIRE |
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