Helicobacter pylori Stress-Response: Definition of the HrcA Regulon
Autor: | Roncarati D1, Pinatel E2, Fiore E3, Peano C4, 5, Loibman S6, Scarlato V7. |
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Přispěvatelé: | Roncarati, Davide, Pinatel, Eva, Fiore, Elisabetta, Peano, Clelia, Loibman, Stefany, Scarlato, Vincenzo |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
Microbiology (medical)
RNA-sequencing Repressor Cellular homeostasis Biology Microbiology Transcriptome 03 medical and health sciences Virology Gene expression Transcriptional regulation Heat shock Gene lcsh:QH301-705.5 030304 developmental biology 0303 health sciences Helicobacter pylori 030306 microbiology Communication stress condition stress conditions heat-shock response Cell biology HrcA repressor Regulon lcsh:Biology (General) helicobacter pylori transcriptome |
Zdroj: | Microorganisms Microorganisms, Vol 7, Iss 10, p 436 (2019) Microorganisms 7 (2019). doi:10.3390/microorganisms7100436 info:cnr-pdr/source/autori:Roncarati D1, Pinatel E2, Fiore E3, Peano C4,5, Loibman S6, Scarlato V7./titolo:Helicobacter pylori Stress-Response: Definition of the HrcA Regulon/doi:10.3390%2Fmicroorganisms7100436/rivista:Microorganisms/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:7 |
DOI: | 10.3390/microorganisms7100436 |
Popis: | Bacteria respond to different environmental stresses by reprogramming the transcription of specific genes whose proper expression is critical for their survival. In this regard, the heat-shock response, a widespread protective mechanism, triggers a sudden increase in the cellular concentration of different proteins, including molecular chaperones and proteases, to preserve protein folding and maintain cellular homeostasis. In the medically important gastric pathogen Helicobacter pylori the regulation of the principal heat-shock genes is under the transcriptional control of two repressor proteins named HspR and HrcA. To define the HrcA regulon, we carried out whole transcriptome analysis through RNA-sequencing, comparing the transcriptome of the H. pylori G27 wild type strain to that of the isogenic hrcA-knockout strain. Overall, differential gene expression analysis outlined 49 genes to be deregulated upon hrcA gene inactivation. Interestingly, besides controlling the transcription of genes coding for molecular chaperones and stress-related mediators, HrcA is involved in regulating the expression of proteins whose function is linked to several cellular processes crucial for bacterial survival and virulence. These include cell motility, membrane transporters, Lipopolysaccharide modifiers and adhesins. The role of HrcA as a central regulator of H. pylori transcriptome, as well as its interconnections with the HspR regulon are here analyzed and discussed. As the HrcA protein acts as a pleiotropic regulator, influencing the expression of several stress-unrelated genes, it may be considered a promising target for the design of new antimicrobial strategies. |
Databáze: | OpenAIRE |
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