Botulinum toxin A and lidocaine have an impact on adipose-derived stem cells, fibroblasts, and mature adipocytes in vitro
| Autor: | Johanna Kober, Stefanie Nickl, Maike Keck, Melanie Schmid, Lars-Peter Kamolz, Alfred Gugerell |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Lidocaine medicine.drug_class Adipose tissue In Vitro Techniques Transplant Donor Site Adipocytes Humans Medicine Anesthetics Local Botulinum Toxins Type A Cells Cultured business.industry Local anesthetic Stem Cells Cell Differentiation Fibroblasts Botulinum toxin In vitro Resorption Surgery Adipose Tissue Adipogenesis Stem cell business Stem Cell Transplantation medicine.drug |
| Zdroj: | Journal of Plastic, Reconstructive & Aesthetic Surgery. 67:1276-1281 |
| ISSN: | 1748-6815 |
| Popis: | Summary Lipofilling with autologous fat tissue is widely used in plastic and reconstructive surgery to treat soft-tissue deficiency. Unfortunately, implanted cells disappear gradually and make it difficult to predict the resorption rate. Several adjuvants are used to improve the success of fat tissue grafting. In this study, the effect of botulinum toxin (BoNT) on mature adipocytes, as well as adipose-derived stem cells (ASC) and fibroblasts was evaluated. As lidocaine is the most prevalent drug to anesthetize the donor site as well as the area to be treated with autologous fat, this local anesthetic was examined too. Primary ASCs, fibroblasts, and mature adipocytes were exposed to 1, 10, and 20 IU/ml BoNT A and 1% lidocaine. Cells were tested on proliferation, viability, and LDH release. Adipogenic differentiation potential was evaluated by quantitative real-time PCR analyzing the expression of FABP4. BoNT had no significant influence on the proliferation or viability of tested cells. By trend, low concentrations of BoNT improved adipogenic potential of ASCs. Lidocaine had a strong diminishing effect on the proliferation of ASCs and fibroblasts and on the viability of these cells. Mature adipocytes show no significant inferior viability after BoNT or lidocaine treatment. BoNT has no negative effect on ASCs, mature adipocytes, or fibroblasts in vitro. Lidocaine (1%) negatively influences the proliferation and viability of fibroblasts and partly of ASCs but not of mature adipocytes. |
| Databáze: | OpenAIRE |
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