GC Content of Early Metazoan Genes and Its Impact on Gene Expression Levels in Mammalian Cell Lines
| Autor: | Yvan Saeys, Ellen Sanders, Rudi Beyaert, Bernd Schierwater, Jens Staal, Kai Kamm, Paco Hulpiau, Ismail Sahin Gul, Frans van Roy, Evi De Keuckelaere, Ulrich Technau, Yasmine Driege, Katrien Staes, Tom Deroo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
SELECTION
0301 basic medicine Genome evolution ectopic ISOCHORES ORGANIZATION genome evolution Cell Line ectopic expression Evolution Molecular 03 medical and health sciences Trichoplax TRICHOPLAX-ADHAERENS HISTORY expression Gene expression Genetics HUMAN GENOME Animals Humans Placozoa Gene Ecology Evolution Behavior and Systematics Phylogeny Mammals SITES GC content COMPLEXITY 030102 biochemistry & molecular biology biology Trichoplax adhaerens Biology and Life Sciences Proteins Paracaspase biology.organism_classification EVOLUTION 030104 developmental biology CADHERINS Human genome Ectopic expression Research Article |
| Zdroj: | Genome Biology and Evolution GENOME BIOLOGY AND EVOLUTION |
| ISSN: | 1759-6653 |
| Popis: | With the genomes available for many animal clades, including the early-branching metazoans, one can readily study the functional conservation of genes across a diversity of animal lineages. Ectopic expression of an animal protein in, for instance, a mammalian cell line is a generally used strategy in structure–function analysis. However, this might turn out to be problematic in case of distantly related species. Here we analyzed the GC content of the coding sequences of basal animals and show its impact on gene expression levels in human cell lines, and, importantly, how this expression efficiency can be improved. Optimization of the GC3 content in the coding sequences of cadherin, alpha-catenin, and paracaspase of Trichoplax adhaerens dramatically increased the expression of these basal animal genes in human cell lines. |
| Databáze: | OpenAIRE |
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