In Vivo Pharmacokinetics, Biodistribution and Toxicity of Antibody-Conjugated Gold Nanoparticles in Healthy Mice
| Autor: | Stéphane Lucas, Carine Michiels, Karen Van Hoecke, Thomas Cardinaels, Noami Daems, Sarah Baatout, Bart Verlinden, An Aerts |
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| Rok vydání: | 2020 |
| Předmět: |
Technology
Biodistribution Materials Science 0206 medical engineering In Vivo Biomedical Engineering Cetuximab Pharmaceutical Science Medicine (miscellaneous) Bioengineering Spleen 02 engineering and technology Pharmacology NANORODS Pharmacokinetics In vivo medicine Gold Nanoparticles VITRO General Materials Science Nanoscience & Nanotechnology Materials Science Biomaterials Kidney Science & Technology Toxicity biology business.industry 021001 nanoscience & nanotechnology 020601 biomedical engineering medicine.anatomical_structure Cancer cell biology.protein Science & Technology - Other Topics PENETRATION Antibody 0210 nano-technology business SYSTEMATIC EVALUATION |
| Zdroj: | Daems, N, Verlinden, B, Van Hoecke, K, Cardinaels, T, Baatout, S, Michiels, C, Lucas, S & Aerts, A 2020, ' In Vivo Pharmacokinetics, Biodistribution and Toxicity of Antibody-Conjugated Gold Nanoparticles in Healthy Mice ', Journal of Biomedical Nanotechnology, vol. 16, no. 6, pp. 985-996 . https://doi.org/10.1166/jbn.2020.2928 |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2020.2928 |
| Popis: | Cetuximab-conjugated gold nanoparticles are known to target cancer cells, but display toxicity towards normal kidney, liver and endothelial cells in vitro. In this study, we investigated their pharmacokinetics, biodistribution and toxicity after intravenous administration in healthy mice. Our data showed that these nanoparticles were rapidly cleared from the blood and accumulated mainly in the liver and spleen with long-term retention. Acute liver injury, inflammatory activity and vascular damage were transient and negligible, as confirmed by the liver functionality tests and serum marker analysis. There was no sign of altered liver, kidney, lung and spleen morphology up to 4 weeks post-injection. After 6 months, kidney casts and splenic apoptosis appeared to be more prevalent than in the controls. Furthermore, occasional immune cell infiltration was observed in the lungs. Therefore, we recommend additional in vivo studies, in order to investigate the long-term toxicity and elimination of gold nanoparticles after multiple dosing in their preclinical validation as new targeted anti-cancer therapies. ispartof: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY vol:16 issue:6 pages:985-996 ispartof: location:United States status: published |
| Databáze: | OpenAIRE |
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