Biohybrid methacrylated gelatin/polyacrylamide hydrogels for cartilage repair
| Autor: | Xiong Lu, Lu Han, Huipin Yuan, Jielong Xu, Wang Zhixiong, Kefeng Wang, Jie Weng, Donglin Gan, Hongping Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Polyacrylamide Hydrogel
food.ingredient Materials science Biocompatibility Biomedical Engineering Methacrylic anhydride macromolecular substances 02 engineering and technology 010402 general chemistry complex mixtures 01 natural sciences Gelatin chemistry.chemical_compound food Tissue engineering General Materials Science Composite material chemistry.chemical_classification technology industry and agriculture General Chemistry General Medicine Dynamic mechanical analysis Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Self-healing hydrogels 0210 nano-technology Biomedical engineering |
| Zdroj: | Journal of materials chemistry. B. 5(4) |
| ISSN: | 2050-7518 |
| Popis: | Articular cartilage defect repair is challenging for clinics because of the lack of self-regenerative ability of avascular tissue. Gelatin-based hydrogels are widely used in the field of tissue engineering because of their good biodegradability, excellent biocompatibility, and cell/tissue affinity. However, gelatin-based hydrogels exhibit poor thermal stability and low mechanical strength, which limit their application in cartilage repair. In this study, methacrylic anhydride (MA) was employed to modify gelatin to obtain photo-crosslinkable methacrylated gelatin (GelMA). The GelMA-based natural–synthetic polymer biohybrid hydrogel was prepared by co-polymerizing acrylamide (AM) and GelMA under ultraviolet radiation in the presence of a photo-initiator. The GelMA/PAM biohybrid hydrogel simultaneously possessed the advantages of both PAM hydrogels and GelMA hydrogels. The GelMA block provided specific biological functions for cell adhesion and proliferation, while the flexible PAM chains reinforced the brittle gelatin network and sustained the load during deformation. Compared with pure PAM hydrogel and GelMA, the GelMA/PAM biohybrid hydrogels showed enhanced compression strength (0.38 MPa) and improved elasticity (storage modulus of 1000 Pa). The GelMA/PAM biohybrid hydrogel showed a favorable degradation rate and sustained protein release. In vitro cell culture showed that the chondrocytes remained viable and proliferated on the biohybrid hydrogel, demonstrating that the biohybrid hydrogels had good cell adhesion and excellent biocompatibility. In a rabbit knee cartilage defect model, we evaluated the cartilage repair ability of the biohybrid hydrogel in vivo. In summary, this study demonstrated that hybridization of synthetic polymers considerably improves the performance and expands the application of the gelatin-based hydrogels. The biohybrid hydrogel is a good candidate material to be applied in articular cartilage tissue engineering and may have great potential in various soft tissue engineering applications. |
| Databáze: | OpenAIRE |
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