Antioxidant activities of aqueous leaf extracts of Toona sinensis on free radical-induced endothelial cell damage

Autor: Hsin Ju Cho, Hsin-Ling Yang, Ssu Ching Chen, K. J. Senthil Kumar, You-Cheng Hseu, Lai Wang, Mei Tsun Wang, Yu Chang Chen, Hui-Chi Huang, Kai Yuan Lin
Rok vydání: 2011
Předmět:
Antioxidant
Toona sinensis
medicine.medical_treatment
Interleukin-1beta
Amidines
Pharmacology
medicine.disease_cause
Antioxidants
chemistry.chemical_compound
Malondialdehyde
Drug Discovery
Gallic acid
Meliaceae
bcl-2-Associated X Protein
ICAM-1
biology
U937 Cells
Catalase
Intercellular Adhesion Molecule-1
Oxidants
Proto-Oncogene Proteins c-bcl-2
Biochemistry
Tissue Plasminogen Activator
E-Selectin
Free Radicals
Cell Survival
Vascular Cell Adhesion Molecule-1
Gallic Acid
Plasminogen Activator Inhibitor 1
Human Umbilical Vein Endothelial Cells
medicine
Humans
Viability assay
Cell damage
Plants
Medicinal
Dose-Response Relationship
Drug
Superoxide Dismutase
Water
biology.organism_classification
medicine.disease
Epoprostenol
Coculture Techniques
Plant Leaves
Oxidative Stress
chemistry
Cytoprotection
Solvents
biology.protein
Oxidative stress
DNA Damage
Drugs
Chinese Herbal
Zdroj: Journal of Ethnopharmacology. 137:669-680
ISSN: 0378-8741
DOI: 10.1016/j.jep.2011.06.017
Popis: Ethnopharmacological relavence In Taiwan, Toona sinensis ( Toona sinensis ) is well known as a traditional Chinese medicine, while the underlying pharmacological mechanisms of this drug are still a matter of debate. Materials and methods The purpose of this study was to evaluate the protective effects of non-cytotoxic concentrations of aqueous leaf extracts of Toona sinensis (TS extracts; 50–100 μg/mL) and gallic acid (5 μg/mL), a major component of these extracts, against AAPH-induced oxidative cell damage in human umbilical vein endothelial cells (ECs). Results Exposure of ECs to AAPH (15 mM) decreased cell viability from 100% to 43%. However, ECs were pre-incubated with TS extracts prior to AAPH induction resulted in increased resistance to oxidative stress and cell viability in a dose-dependent manner. An increase in ECs-derived PGI 2 and IL-1β in response to AAPH exposure was positively correlated with cytotoxicity and negatively with TS extracts concentrations. In addition, gallic acid also suppressed PGI 2 and IL-1β production in AAPH-induced ECs. Notably, TS extracts/gallic acid treatment significantly inhibited ROS generation, MDA formation, SOD/catalase activity, and Bax/Bcl-2 dysregulation in AAPH-stimulated ECs. Pretreatment of ECs with TS extracts/gallic acid also suppressed AAPH-induced cell surface expression and secretion of VCAM-1, ICAM-1 and E-selectin, which was associated with abridged adhesion of U937 leukocytes to ECs. Moreover, TS extracts/gallic acid treatment significantly inhibited the AAPH-mediated up regulation of PAI-1 and down regulation of t-PA in ECs, which may decrease fibrinolytic activity. Conclusions Therefore, Toona sinensis may possess antioxidant properties that protect endothelial cells from oxidative stress. Our results also support the traditional use of Toona sinensis in the treatment of free radical-related diseases and atherosclerosis.
Databáze: OpenAIRE
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