Genome-wide analysis of EgEVE_1, a transcriptionally active endogenous viral element associated to small RNAs in Eucalyptus genomes
| Autor: | Juliana Costa-Silva, Douglas Silva Domingues, Celso Luis Marino, Helena Sanches Marcon, Alan P. R. Lorenzetti |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Tecnológica Federal do Paraná, Universidade Estadual de Londrina (UEL) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Plant Molecular Biology Genome Pararetrovirus insertion 03 medical and health sciences Caulimovirus Botany Genetics horizontal transfer Molecular Biology Eucalyptus Phylogenetic tree biology fungi biology.organism_classification lcsh:Genetics 030104 developmental biology Horizontal gene transfer Caulimoviridae Mobile genetic elements Endogenous viral element |
| Zdroj: | Genetics and Molecular Biology, Volume: 40, Issue: 1 Supplement 1, Pages: 217-225, Published: 23 FEB 2017 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Genetics and Molecular Biology v.40 n.1 suppl.1 2017 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Vol 40, Iss 1 suppl 1, Pp 217-225 (2017) |
| ISSN: | 1415-4757 |
| Popis: | Made available in DSpace on 2018-12-11T17:32:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01. Added 1 bitstream(s) on 2021-07-15T15:04:15Z : No. of bitstreams: 1 S1415-47572017000200217.pdf: 2227310 bytes, checksum: ff9948fb5bb58d84242606d224b5e35a (MD5) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Endogenous viral elements (EVEs) are the result of heritable horizontal gene transfer from viruses to hosts. In the last years, several EVE integration events were reported in plants by the exponential availability of sequenced genomes. Eucalyptus grandis is a forest tree species with a sequenced genome that is poorly studied in terms of evolution and mobile genetic elements composition. Here we report the characterization of E. grandis endogenous viral element 1 (EgEVE_1), a transcriptionally active EVE with a size of 5,664 bp. Phylogenetic analysis and genomic distribution demonstrated that EgEVE_1 is a newly described member of the Caulimoviridae family, distinct from the recently characterized plant Florendoviruses. Genomic distribution of EgEVE_1 and Florendovirus is also distinct. EgEVE_1 qPCR quantification in Eucalyptus urophylla suggests that this genome has moreEgEVE_1 copies than E. grandis. EgEVE_1 transcriptional activity was demonstrated by RT-qPCR in five Eucalyptus species and one intrageneric hybrid. We also identified that Eucalyptus EVEs can generate small RNAs (sRNAs),that might be involved in de novo DNA methylation and virus resistance. Our data suggest that EVE families in Eucalyptus have distinct properties, and we provide the first comparative analysis of EVEs in Eucalyptus genomes. Departamento de Botânica Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Departamento de Genética Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) PPGBIOINFO Universidade Tecnológica Federal do Paraná Genética e Biologia Molecular Universidade Estadual de Londrina Departamento de Botânica Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Departamento de Genética Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) CNPq: 474123/2010-3 |
| Databáze: | OpenAIRE |
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