Screening of mutants using chlorophyll fluorescence
| Autor: | Takako Ogawa, Kintake Sonoike |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chlorophyll
0106 biological sciences 0301 basic medicine Research Mutant Plant physiology Plant Science Biology Photosynthesis 01 natural sciences Fluorescence 03 medical and health sciences Spectrometry Fluorescence 030104 developmental biology Plant biochemistry Screening method Biophysics Chlorophyll fluorescence 010606 plant biology & botany |
| Zdroj: | Journal of Plant Research. 134:653-664 |
| ISSN: | 1618-0860 0918-9440 |
| DOI: | 10.1007/s10265-021-01276-6 |
| Popis: | Chlorophyll fluorescence has been widely used for the estimation of photosynthesis or its regulatory mechanisms. Chlorophyll fluorescence measurements are the methods with non-destructive nature and do not require contact between plant materials and fluorometers. Furthermore, the measuring process is very rapid. These characteristics of chlorophyll fluorescence measurements make them a suitable tool to screen mutants of photosynthesis-related genes. Furthermore, it has been shown that genes with a wide range of functions can be also analyzed by chlorophyll fluorescence through metabolic interactions. In this short review, we would like to first introduce the basic principle of the chlorophyll fluorescence measurements, and then explore the advantages and limitation of various screening methods. The emphasis is on the possibility of chlorophyll fluorescence measurements to screen mutants defective in metabolisms other than photosynthesis. |
| Databáze: | OpenAIRE |
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