Vimentin and laminin are altered on cheek pouch microvessels of streptozotocin-induced diabetic hamsters
| Autor: | Jemima F. R. da Silva, Fatima Z. G. A. Cyrino, Jorge José de Carvalho, Eliete Bouskela, M. M. D. Breitenbach |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Cell Membrane Permeability Time Factors Endothelium Vasodilator Agents Vimentin Microcirculation Diabetes Mellitus Experimental Type IV collagen Random Allocation Cheek pouch Laminin Internal medicine Cricetinae medicine Diabetes Mellitus Animals Microvessel Cytoskeleton lcsh:R5-920 biology Mesocricetus General Medicine Streptozotocin Immunohistochemistry Acetylcholine Extracellular Matrix Arterioles Disease Models Animal medicine.anatomical_structure Endocrinology Basic Research Cheek Immunology Microvessels biology.protein lcsh:Medicine (General) medicine.drug Histamine |
| Zdroj: | Clinics Clinics; v. 66 n. 11 (2011); 1961-1968 Clinics; Vol. 66 Núm. 11 (2011); 1961-1968 Clinics; Vol. 66 No. 11 (2011); 1961-1968 Universidade de São Paulo (USP) instacron:USP Clinics, Vol 66, Iss 11, Pp 1961-1968 (2011) Clinics, Volume: 66, Issue: 11, Pages: 1961-1968, Published: 2011 |
| ISSN: | 1980-5322 1807-5932 |
| Popis: | OBJECTIVE: Normal endothelial cells respond to shear stress by elongating and aligning in the direction of fluid flow. Hyperglycemia impairs this response and contributes to microvascular complications, which result in deleterious effects to the endothelium. This work aimed to evaluate cheek pouch microvessel morphological characteristics, reactivity, permeability, and expression of cytoskeleton and extracellular matrix components in hamsters after the induction of diabetes with streptozotocin. METHODS: Syrian golden hamsters (90-130 g) were injected with streptozotocin (50 mg/kg, i.p.) or vehicle either 6 (the diabetes mellitus 6 group) or 15 (the diabetes mellitus 15 group) days before the experiment. Vascular dimensions and density per area of vessels were determined by morphometric and stereological measurements. Changes in blood flow were measured in response to acetylcholine, and plasma extravasation was measured by the number of leakage sites. Actin, talin, α-smooth muscle actin, vimentin, type IV collagen, and laminin were detected by immunohistochemistry and assessed through a semiquantitative scoring system. RESULTS: There were no major alterations in the lumen, wall diameters, or densities of the examined vessels. Likewise, vascular reactivity and permeability were not altered by diabetes. The arterioles demonstrated increased immunoreactivity to vimentin and laminin in the diabetes mellitus 6 and diabetes mellitus 15 groups. DISCUSSION: Antibodies against laminin and vimentin inhibit branching morphogenesis in vitro. Therefore, laminin and vimentin participating in the structure of the focal adhesion may play a role in angiogenesis. CONCLUSIONS: Our results indicated the existence of changes related to cell-matrix interactions, which may contribute to the pathological remodeling that was already underway one week after induction of experimental diabetes. |
| Databáze: | OpenAIRE |
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