Variation among S-locus haplotypes and among stylar RNases in almond
| Autor: | Maria Hrmova, Adam E. Croxford, Shashi N. Goonetilleke, Timothy J. March, Diane E. Mather, Michelle Wirthensohn |
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| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular 0106 biological sciences 0301 basic medicine Glycosylation lcsh:Medicine Locus (genetics) Biology 01 natural sciences Article Protein Structure Secondary Self incompatability 03 medical and health sciences Complete sequence Ribonucleases Plant hybridization Catalytic Domain Allele lcsh:Science Gene Plant Proteins 2. Zero hunger Genetics Genetic diversity Multidisciplinary F-Box Proteins lcsh:R Haplotype Terminal Repeat Sequences Sequence Analysis DNA Prunus dulcis Long terminal repeat Hypervariable region 030104 developmental biology Haplotypes Genetic Loci lcsh:Q 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | In many plant species, self-incompatibility systems limit self-pollination and mating among relatives. This helps maintain genetic diversity in natural populations but imposes constraints in agriculture and plant breeding. In almond [Prunus dulcis (Mill.) D.A. Webb], the specificity of self-incompatibility is mainly determined by stylar ribonuclease (S-RNase) and S-haplotype-specific F-box (SFB) proteins, both encoded within a complex locus, S. Prior to this research, a nearly complete sequence was available for one S-locus haplotype. Here, we report complete sequences for four haplotypes and partial sequences for 11 haplotypes. Haplotypes vary in sequences of genes (particularly S-RNase and SFB), distances between genes and numbers and positions of long terminal repeat transposons. Haplotype variation outside of the S-RNase and SFB genes may help maintain functionally important associations between S-RNase and SFB alleles. Fluorescence-based assays were developed to distinguish among some S-RNase alleles. With three-dimensional modelling of five S-RNase proteins, conserved active sites were identified and variation was observed in electrostatic potential and in the numbers, characteristics and positions of secondary structural elements, loop anchoring points and glycosylation sites. A hypervariable region on the protein surface and differences in the number, location and types of glycosylation sites may contribute to determining S-RNase specificity. |
| Databáze: | OpenAIRE |
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