Chromatin architecture transitions from zebrafish sperm through early embryogenesis
| Autor: | Aneasha F. Whittaker-Tademy, Mengyao Tan, Erez Lieberman Aiden, Hiroyuki Takeda, Bradley R. Cairns, Noelia Díaz, Neva C. Durand, Candice L. Wike, Dana Klatt Shaw, David Grunwald, Ryohei Nakamura, Yixuan Guo, Juan M. Vaquerizas |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
animal structures Zygote Embryonic Development Genome Chromosomes 03 medical and health sciences 0302 clinical medicine Genetics medicine Animals Enhancer Mitosis Zebrafish Genetics (clinical) 030304 developmental biology 0303 health sciences biology Research Gene Expression Regulation Developmental biology.organism_classification Spermatozoa Chromatin Cell biology Histone medicine.anatomical_structure biology.protein Maternal to zygotic transition Gamete 030217 neurology & neurosurgery |
| Zdroj: | Genome Res |
| ISSN: | 1549-5469 1088-9051 |
| DOI: | 10.1101/gr.269860.120 |
| Popis: | Chromatin architecture mapping in 3D formats has increased our understanding of how regulatory sequences and gene expression are connected and regulated in a genome. The 3D chromatin genome shows extensive remodeling during embryonic development, and although the cleavage-stage embryos of most species lack structure before zygotic genome activation (pre-ZGA), zebrafish has been reported to have structure. Here, we aimed to determine the chromosomal architecture in paternal/sperm zebrafish gamete cells to discern whether it either resembles or informs early pre-ZGA zebrafish embryo chromatin architecture. First, we assessed the higher-order architecture through advanced low-cell in situ Hi-C. The structure of zebrafish sperm, packaged by histones, lacks topological associated domains and instead displays “hinge-like” domains of ∼150 kb that repeat every 1–2 Mbs, suggesting a condensed repeating structure resembling mitotic chromosomes. The pre-ZGA embryos lacked chromosomal structure, in contrast to prior work, and only developed structure post-ZGA. During post-ZGA, we find chromatin architecture beginning to form at small contact domains of a median length of ∼90 kb. These small contact domains are established at enhancers, including super-enhancers, and chemical inhibition of Ep300a (p300) and Crebbpa (CBP) activity, lowering histone H3K27ac, but not transcription inhibition, diminishes these contacts. Together, this study reveals hinge-like domains in histone-packaged zebrafish sperm chromatin and determines that the initial formation of high-order chromatin architecture in zebrafish embryos occurs after ZGA primarily at enhancers bearing high H3K27ac. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|