20-HETE-promoted cerebral blood flow autoregulation is associated with enhanced pericyte contractility
| Autor: | Baoying Zheng, Xing Fang, Celeste Yc. Wu, Jane J Ryu, Richard J. Roman, Fan Fan, Huawei Zhang, Yedan Liu, Tina Yu, Zongbo Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Vascular smooth muscle Physiology 030204 cardiovascular system & hematology Biochemistry Cerebral autoregulation Mural cell Article Contractility 03 medical and health sciences 0302 clinical medicine medicine.artery Internal medicine Hydroxyeicosatetraenoic Acids medicine Autoregulation Pharmacology Chemistry Cell Biology 030104 developmental biology medicine.anatomical_structure Endocrinology Cerebral blood flow Middle cerebral artery cardiovascular system Pericyte Pericytes |
| Zdroj: | Prostaglandins Other Lipid Mediat |
| ISSN: | 1098-8823 |
| Popis: | We previously reported that deficiency in 20-HETE or CYP4A impaired the myogenic response and autoregulation of cerebral blood flow (CBF) in rats. The present study demonstrated that CYP4A was coexpressed with alpha-smooth muscle actin (α-SMA) in vascular smooth muscle cells (VSMCs) and most pericytes along parenchymal arteries (PAs) isolated from SD rats. Cell contractile capabilities of cerebral VSMCs and pericytes were reduced with a 20-HETE synthesis inhibitor, HET0016, but restored with 20-HETE analog WIT003. Similarly, intact myogenic responses of the middle cerebral artery and PA of SD rats decreased with HET0016 and were rescued by WIT003. The myogenic response of the PA was abolished in SS and was restored in SS.BN5 and SS.Cyp4a1 rats. HET0016 enhanced CBF and impaired its autoregulation in the surface and deep cortex of SD rats. These results demonstrate that 20-HETE has a direct effect on cerebral mural cell contractility that may play an essential role in controlling cerebral vascular function. |
| Databáze: | OpenAIRE |
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